Q7VWV5 (QUEF_BORPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	BP2084

Organism

Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) [Reference proteome] [HAMAP]

Taxonomic identifier

257313 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella › Bordetella pertussis

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817		PRO_0000163022

Sequences

	Sequence		Length	Mass (Da)
	Q7VWV5 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 2746EE8D2556FA78	FASTA	273	30,621
	10 20 30 40 50 60 MSLSNAPLGQ HVAYPSQYDP GLLFPIPRAT NRASLQLGAA LPFTGVDLWN AYELSWLDAR 70 80 90 100 110 120 GKPRVAMATF SFPADSPNIV ESKSFKLYLN SFNQTRLPNA QALRDRLERD LAAAAGAPVG 130 140 150 160 170 180 LEFISPQRFG ELNMAELDGI YIDKLDIEID TYEPAPQLLQ CAPGDEVEET LATRLLKSNC 190 200 210 220 230 240 PVTGQPDWAS LQVRYRGRPI DRAALLKYVV SFRQHAEFHE HCVERIFGDI MRACQPRQLT 250 260 270 VYARYTRRGG LDINPWRSNF ESAPPADVRT ARQ

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tohama I / ATCC BAA-589 / NCTC 13251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640417 Genomic DNA. Translation: CAE42362.1.
RefSeq	NP_880745.1. NC_002929.2.
3D structure databases
ProteinModelPortal	Q7VWV5.
SMR	Q7VWV5. Positions 14-273.
ModBase	Search...
Protein-protein interaction databases
STRING	257313.BP2084.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE42362; CAE42362; BP2084.
GeneID	2667196.
KEGG	bpe:BP2084.
PATRIC	21157506. VBIBorPer7866_2241.
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HOG000273755.
KO	K06879.
OMA	ERIFCDL.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	BPER257313:BP2084-MONOMER.
UniPathway	UPA00392.
Family and domain databases
HAMAP	MF_00817. QueF_type2.
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BORPE

Accession

Primary (citable) accession number: Q7VWV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program