Reviewed, UniProtKB/Swiss-Prot Q7VUL8 (SAHH_BORPE)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Synonyms:	acyH
Ordered Locus Names:	BP3068

Organism

Bordetella pertussis [Complete proteome] [HAMAP]

Taxonomic identifier

520 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000116949
Regions
	Region	224 – 389	166	NAD binding By similarity
Sites
	Binding site	62	1	Substrate By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
	Binding site	231	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VUL8-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: B2F571BFCE71D308	FASTA	472	51,631
	10 20 30 40 50 60 MNAVTDKSVA DYIVADMALA GWGRRELAIA ETEMPGLMAI RDEYAASQPL KGARIAGSLH 70 80 90 100 110 120 MTIQTGVLIE TLVALGAEVR WASCNIFSTQ DHAAAAIAAT GTPVFAIKGE TLEEYWQYTH 130 140 150 160 170 180 KIFEWPEGRH ANMILDDGGD ATLLLHLGAR AEQDISVLAK PGSEEERVLF AAIKETLGRD 190 200 210 220 230 240 PKWYSTRLAQ IKGVTEETTT GVHRLYQMSQ KGELAFAAIN VNDSVTKSKF DNLYGCRESL 250 260 270 280 290 300 VDGIKRATDV MVAGKIAVVA GYGDVGKGCA QALVALRAQV WVTEIDPICA LQAAMEGFKV 310 320 330 340 350 360 VTMEEAAAHA DIFVTATGNY HVITRQHMEA MKDQAIVCNI GHFDNEIDVA GLENCQWEEI 370 380 390 400 410 420 KPQVDHVIFP DGKRIILLAK GRLVNLGCAT GHPSFVMSSS FANQTIAQIE LFTRNEAYTT 430 440 450 460 470 GQVYVLPKHL DEKVARLHLK KLGVKLSTLS KQQADYIGVP VEGPFKPDHY RY

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tohama I / ATCC BAA-589 / NCTC 13251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640420 Genomic DNA. Translation: CAE43337.1.
RefSeq	NP_881639.1.
3D structure databases
SMR	Q7VUL8. Positions 11-472.
ModBase	Search...
Genome annotation databases
GeneID	2665925.
KEGG	bpe:BP3068.
NMPDR	fig|257313.1.peg.2711.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352029.
OMA	VITHDHM.
PhylomeDB	Q7VUL8.
Enzyme and pathway databases
BioCyc	BPER257313:BP3068-MONOMER.
BRENDA	3.3.1.1. 21511.
Family and domain databases
HAMAP	MF_00563_B. AdoHcyase_B. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_BORPE

Accession

Primary (citable) accession number: Q7VUL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)