Reviewed, UniProtKB/Swiss-Prot Q7VU94 (SYQ_BORPE)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	BP3226

Organism

Bordetella pertussis [Complete proteome] [HAMAP]

Taxonomic identifier

520 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 587	587	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000095481
Regions
	Motif	58 – 68	11	"HIGH" region HAMAP MF_00126
	Motif	301 – 305	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	304	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VU94-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 096458360E042B7D	FASTA	587	66,585
	10 20 30 40 50 60 MTHAPTPPAA SNFLRPIIED DLQANRFQGK LWAGKPGPAA LQAQGQPDPA RIRTRFPPEP 70 80 90 100 110 120 NGYLHIGHAK SICVNFGLAR DYGGVCHLRF DDTNPEKEEQ EYVDAIIEAV HWLGFDWQAD 130 140 150 160 170 180 GNDNLYFASD YFEFMYEFAE ALVQAGHAYV DEQSAEEIRA SRGTLTEPGT DSPWRDRPAD 190 200 210 220 230 240 ESLLRLREMR DGKHPDGSLV LRARIDMASP NINLRDPVMY RVRHATHHRT GNAWCIYPMY 250 260 270 280 290 300 SWAHPVEDAL EGITHSICTL EFEDQRPFYD WILARLAELG KLARPLPHQY EFARLNLTYV 310 320 330 340 350 360 VTSKRKLLQL VREGYVDGWD DPRMPTLFGL RRRGYTPSSI RLFCDRTAVS KSDSRIDYSL 370 380 390 400 410 420 LEQAVRDDLD PVAPRSVAVL DPLKLVITNY PEGRSETCSA PRNPHDPQAG VREFPFTREL 430 440 450 460 470 480 WIEQDDFREE PPKKYFRLFP GNTVRLKYGY VVRCTGFTKD ESGKVVEVQA EYLPDTKSGT 490 500 510 520 530 540 PGADSVKVKG NITWVSAAHA VPAQVHLYDR LFADAHPDGG DKDFLACLNP NSKQTVQAWL 550 560 570 580 EPGIEAVPGA TWQFERLGYF TVDSKDSRPE APVLNRIVTL RDSWQAA

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tohama I / ATCC BAA-589 / NCTC 13251.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640420 Genomic DNA. Translation: CAE43492.1.
RefSeq	NP_881776.1.
3D structure databases
HSSP	HSSP built from PDB template 1GTR based on UniProtKB P00962.
ModBase	Search...
Genome annotation databases
GeneID	2667499.
GenomeReviews	Gene locus BP3226 in contig BX470248_GR.
KEGG	bpe:BP3226.
NMPDR	fig|257313.1.peg.2847.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VU94.
OMA	NFLRQII.
Enzyme and pathway databases
BioCyc	BPER257313:BP3226-MON.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_BORPE

Accession

Primary (citable) accession number: Q7VU94

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)