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Q7VTR9 (PYRD_BORPE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydroorotate dehydrogenase (quinone)
EC=1.3.5.2
Alternative name(s):
DHOdehase
Short name=DHOD
Short name=DHODase
Dihydroorotate oxidase
Gene names
Name:pyrD
Ordered Locus Names:BP3442
OrganismBordetella pertussis
Taxonomic identifier520 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor By similarity. HAMAP MF_00225

Catalytic activity

(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP MF_00225

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225

Subunit structure

Monomer By similarity. HAMAP MF_00225

Subcellular location

Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   Cellular componentCell membrane
Membrane
   LigandFMN
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological process'de novo' pyrimidine base biosynthetic process

Inferred from electronic annotation. Source: InterPro

UMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydroorotate oxidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Dihydroorotate dehydrogenase (quinone) HAMAP MF_00225
PRO_0000148425

Regions

Nucleotide binding67 – 715FMN By similarity
Nucleotide binding326 – 3272FMN By similarity
Region116 – 1205Substrate binding By similarity
Region254 – 2552Substrate binding By similarity

Sites

Active site1831Nucleophile By similarity
Binding site711Substrate By similarity
Binding site911FMN; via amide nitrogen By similarity
Binding site1471FMN By similarity
Binding site1801FMN By similarity
Binding site1801Substrate By similarity
Binding site1851Substrate By similarity
Binding site2251FMN By similarity
Binding site2531FMN; via carbonyl oxygen By similarity
Binding site2761FMN; via amide nitrogen By similarity
Binding site3051FMN; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VTR9 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 5097A29FD68EB850

FASTA34937,116
        10         20         30         40         50         60 
MSILFHAYPL ARPALFAMDA ETAHEVTLAQ LQRAYDCGLT RKLLHAQPEA PATLMGLSLR 

        70         80         90        100        110        120 
NPVGLAAGLD KNGAHIDALG NLGFGFVEVG TVTPRAQPGN PKPRMFRLPR ANALINRLGF 

       130        140        150        160        170        180 
NNQGLDAFIA NVQRSQWRSQ GRILGLNIGK NADTPIERAA EDYLIGLAGV YPHADYVTVN 

       190        200        210        220        230        240 
ISSPNTKNLR ALQGGDELSA LLGQLQERRL ALADQHQRHV PLAVKIAPDL SDDQIDAIAE 

       250        260        270        280        290        300 
ILPRHGIDGV IATNTTLARD AVQGLPHAEE AGGVSGAPVH ELSLRVIERL RSRLGDAVAI 

       310        320        330        340 
IGVGGILSGR QASEKMAAGA QAVQLYTGLI YRGPALVGEC VRALAQGSR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX640421 Genomic DNA. Translation: CAE43705.1.
RefSeqNP_881968.1. NC_002929.2.

3D structure databases

ProteinModelPortalQ7VTR9.
SMRQ7VTR9. Positions 8-344.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2666939.
GenomeReviewsGene locus BP3442 in contig BX470248_GR.
KEGGbpe:BP3442.
NMPDRfig|257313.1.peg.3038.
PATRIC21160512. VBIBorPer7866_3727.

Phylogenomic databases

HOGENOMHBG351027.
OMASYVTVNI.
PhylomeDBQ7VTR9.
ProtClustDBPRK05286.

Enzyme and pathway databases

BioCycBPER257313:BP3442-MONOMER.

Family and domain databases

HAMAPMF_00225. DHO_dh_type2.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK00226.
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsTIGR01036. PyrD_sub2. 1 hit.
PROSITEPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRD_BORPE
AccessionPrimary (citable) accession number: Q7VTR9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2003
Last modified: January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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