Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q7VT61 (TRMB_BORPE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BP3692
OrganismBordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) [Reference proteome] [HAMAP]
Taxonomic identifier257313 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171303

Regions

Region233 – 2364Substrate binding By similarity

Sites

Active site1621 By similarity
Binding site871S-adenosyl-L-methionine By similarity
Binding site1121S-adenosyl-L-methionine By similarity
Binding site1391S-adenosyl-L-methionine By similarity
Binding site1621S-adenosyl-L-methionine By similarity
Binding site1661Substrate By similarity
Binding site1981Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VT61 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 7B5D83B15EB4814F

FASTA25428,051
        10         20         30         40         50         60 
MNTNTPAHPP EGAPLSEATQ AALASAEHAP DSPGATHIRS FVHRRGHITQ RQRDALEQLM 

        70         80         90        100        110        120 
GKWSVPYAPR PLDMAAAFGR QAPTILEIGF GMGETTEKIA LARPGDNFLG VEVFNAGVGS 

       130        140        150        160        170        180 
LLHRIEESAI SNLRIVQHDA VEVVRDMIAP DSLAGVHVYF PDPWPKKRHH KRRLLQPPFV 

       190        200        210        220        230        240 
ALLASRLAPG GYLHCATDWE DYAVQMLEVL GGEPLLRNTA DGYAPRPDFR PQTKFETRGL 

       250 
RLGHGVWDLM FKRA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX640422 Genomic DNA. Translation: CAE43949.1.
RefSeqNP_882197.1. NC_002929.2.

3D structure databases

ProteinModelPortalQ7VT61.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257313.BP3692.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE43949; CAE43949; BP3692.
GeneID2664936.
KEGGbpe:BP3692.
PATRIC21161058. VBIBorPer7866_3994.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMATEILQDC.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycBPER257313:BP3692-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BORPE
AccessionPrimary (citable) accession number: Q7VT61
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways