Q7VRX4 (SYM_BLOFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	Bfl471

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Monomer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Methionine--tRNA ligase HAMAP MF_00098		PRO_0000139116
Regions
	Motif	13 – 23	11	"HIGH" region HAMAP MF_00098
	Motif	330 – 334	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	144	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	157	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity
	Binding site	333	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VRX4 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: E50C74DE58D4F7E5	FASTA	545	63,835
	10 20 30 40 50 60 MPNKKILVTC ALPYANGPLH IGHMLEHIQA DIWVRYQRMK NNEVYFICAD DAHGTAIMLK 70 80 90 100 110 120 SKQLNINPEI MIAKIQQEHY QDCCDFNISY NNYHSTHSEE NYELLNSIYT KLKKSGFIKS 130 140 150 160 170 180 RFISQLYDAQ NNIFLPDRFI IGTCPKCRAH NQNGDNCDQC GAIYTPLDLI NPKSAISGKS 190 200 210 220 230 240 PIIKKSKHLF FDLPQFTKSL YTWIHSGVLQ EETLHKVKEW FKLGLKEWDI SRNEPYFGFK 250 260 270 280 290 300 IPNTSSKYFY VWMDAPIGYM GTFKNLCKKN KNILFEDFWS INSKVELYHF IGKDITYFHS 310 320 330 340 350 360 LFWPAILESC QYRKPTGIFV HGHVTLNGSK ISKSKNNFIN VRTYLSHLNS DYLRYYYATK 370 380 390 400 410 420 LSSKINDIDL NFNDFMLKVN ADIINKILNL ASRNSNFITQ YNYGYLSNSL ENSNLYDFFI 430 440 450 460 470 480 DAQYLIGKLF QQREFSEAMR KIMQLADKAN QYIDKQAPWH IVKQHGIHNK NIALCIFSMG 490 500 510 520 530 540 IQLFRVLVIY LKPVLPILSQ YSEKFLNTRL SWNNINIPLT NHKINKFDII FHRINTNQIS SIINK

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248583 Genomic DNA. Translation: CAD83160.1.
RefSeq	NP_878754.1. NC_005061.1.
3D structure databases
ProteinModelPortal	Q7VRX4.
SMR	Q7VRX4. Positions 3-544.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VRX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1499671.
GenomeReviews	Gene locus Bfl471 in contig BX248583_GR.
KEGG	bfl:Bfl471.
PATRIC	31964553. VBICanBlo38691_0459.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HBG721667.
OMA	CGNQLDP.
PhylomeDB	Q7VRX4.
ProtClustDB	PRK12268.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL471-MONOMER.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR014758. Met-tRNA_synth. IPR023458. Met-tRNA_synth_1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BLOFL

Accession

Primary (citable) accession number: Q7VRX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program