Reviewed, UniProtKB/Swiss-Prot Q7VRX1 (DDL_BLOFL)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Synonyms:	ddlA
Ordered Locus Names:	Bfl474

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	D-alanine--D-alanine ligase		PRO_0000177801
Regions
	Domain	140 – 344	205	ATP-grasp
	Nucleotide binding	170 – 225	56	ATP By similarity
Sites
	Metal binding	298	1	Magnesium or manganese 1 By similarity
	Metal binding	311	1	Magnesium or manganese 1 By similarity
	Metal binding	311	1	Magnesium or manganese 2 By similarity
	Metal binding	313	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VRX1-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 890889A160821F49	FASTA	354	40,241
	10 20 30 40 50 60 MKSKLRVGLV CGGCSLEHEV SLKSAIYIMK YINKRFFEVV VLWISKQGDW YIVENDDMHI 70 80 90 100 110 120 DFFVNKENLH KCMPILMFSS GNRCFEYINN ILSLDVIFPI IHGALGEDGS LQGLLRLMNL 130 140 150 160 170 180 PYVGSDVLGS AICMDKDITK RLLRDSGLSI TPFRTFLAHE KKKLKFLNFA DMFGLPFFVK 190 200 210 220 230 240 PVNQGSSIGV AKVNDDYSFH SALDIAFFYS HKIIIESCIA GRELECAVLG NNENPITSVC 250 260 270 280 290 300 GEIIKKNDDF YTYCDKYVDH NSEIVIPAVL EKSVSSKIRS IAIRVFQILN CFGMARVDIF 310 320 330 340 350 LFGKDRIIIN EVNTLPGFTS DSMYLKLWQA TGINISTLLT RLIMLALDRY YKYN

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83163.1.
RefSeq	NP_878757.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1499674.
GenomeReviews	Gene locus Bfl474 in contig BX248583_GR.
KEGG	bfl:Bfl474.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VRX1.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL474-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_BLOFL

Accession

Primary (citable) accession number: Q7VRX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)