Reviewed, UniProtKB/Swiss-Prot Q7VRU9 (PDXB_BLOFL)

Names and origin

Protein names

Recommended name:
    Erythronate-4-phosphate dehydrogenase
    EC=1.1.1.290

Gene names

Name:	pdxB
Ordered Locus Names:	Bfl497

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate By similarity.
Catalytic activity	4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH. HAMAP MF_01825
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5. HAMAP MF_01825
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-phosphoerythronate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Erythronate-4-phosphate dehydrogenase HAMAP MF_01825		PRO_0000075973
Sites
	Active site	209	1	By similarity
	Active site	238	1	By similarity
	Active site	255	1	Proton donor By similarity
	Binding site	45	1	Substrate By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	146	1	NAD By similarity
	Binding site	233	1	NAD By similarity
	Binding site	258	1	NAD; via amide nitrogen By similarity
	Binding site	259	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VRU9-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 1DB671FF109F5486	FASTA	372	42,534
	10 20 30 40 50 60 MRILADQAIP YVYKLFGSDN YVQVCDGRSI SANMLKDVDV LIIRSITKVN HMLLYDSSIK 70 80 90 100 110 120 FIGTVTSGVD HIDQNYLKNN NIRCVSTPGS NAVSVVEYVC ATLFWLAQRD CFFLRDKTVG 130 140 150 160 170 180 IIGVGNIGNL LYQRLNSLGV HTLLYDPYKS KCDTDRMSWK SLDILVSKSD ILTLHVPLTY 190 200 210 220 230 240 TGAYPTWHMI NKDILDALPS NSILINTSRG AVVNNDDLLA ILRCGKKINV ILDVWESEPK 250 260 270 280 290 300 LSLPLLSYVD IGTAHIAGYS FESRIRSIKK IYDDYCDYFN VKNKVNWISL GLSDIRYIAV 310 320 330 340 350 360 SRLDECIINR LIQLVYNVYY DHIALKNNVL RLRGFDKLRE YYCFRREWSS LLVDSKNGYN 370 NDILFKFGFS TL

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83186.1.
RefSeq	NP_878780.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1499697.
GenomeReviews	Gene locus Bfl497 in contig BX248583_GR.
KEGG	bfl:Bfl497.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VRU9.
OMA	Q7VRU9. SAPGCNA.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL497-MON.
Family and domain databases
HAMAP	MF_01825. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDXB_BLOFL

Accession

Primary (citable) accession number: Q7VRU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)