Reviewed, UniProtKB/Swiss-Prot Q7VRU2 (DNLJ_BLOFL)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	Bfl507

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity.
Catalytic activity	NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity.
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (NAD+) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	DNA ligase HAMAP MF_01588		PRO_0000313141
Regions
	Nucleotide binding	32 – 36	5	NAD By similarity
	Nucleotide binding	81 – 82	2	NAD By similarity
Sites
	Active site	120	1	N6-AMP-lysine intermediate By similarity
	Metal binding	417	1	Zinc By similarity
	Metal binding	420	1	Zinc By similarity
	Metal binding	436	1	Zinc By similarity
	Metal binding	442	1	Zinc By similarity
	Binding site	118	1	NAD By similarity
	Binding site	141	1	NAD By similarity
	Binding site	181	1	NAD By similarity
	Binding site	299	1	NAD By similarity
	Binding site	323	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VRU2-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 08C8100916151F5E	FASTA	594	67,657
	10 20 30 40 50 60 MKLIKNRVQI LRERLNYLEY LYYVKSESKV SDEEYDILLE ELRQLEFKYP YLITKSSSTQ 70 80 90 100 110 120 SISNVCDNSL NRIRHQKPML SLNSIRDKSQ LLLFDKRIKY KLSNHYNINQ NLIVYCCELK 130 140 150 160 170 180 IDGVALSILY KQGKLVHAAT RGDGKIGENV TKNVNAIDSI PKNLKKNNNE ILPDLLEIRG 190 200 210 220 230 240 EIFISKLSFS KLNQEMLSSG KKVFSNARNA ASGSLRQLDP HVTELRSLVF CCYGISYYAG 250 260 270 280 290 300 LKQLPNSHWG RLKLCNRWGL SINNYIQIVS GIDPVLEYYN YVRKIRSELE YQIDGIVIKI 310 320 330 340 350 360 DNCIYQDQLG CGFRAPNWAV AYKFPAELKL TKLNDVVFKV GRTGLITPIA YINPVVIGNV 370 380 390 400 410 420 IIKKVNMHNV NEVKRLNLMI GDTVIVQRSG DVIPKILKII LENRVDNAKF IKIPEFCPSC 430 440 450 460 470 480 GSILKVEDNK SSVLRCLAKL TCLAQRKSAL KHFASRKAMN IQGMGSRVIN QLVDKGLICT 490 500 510 520 530 540 PVDFFSLSKE KLLCLDRYGE KSAERLLQSI LIAKEITLSA FIYALGIPGV GESVSNKLAY 550 560 570 580 590 MYRTIENLMN ADLQSFLQLK FIGKIAAMNI YSFLHQSDNY NNIQGLIRSN LIFK

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83193.1.
RefSeq	NP_878787.1.
3D structure databases
HSSP	HSSP built from PDB template 1B04 based on UniProtKB O87703.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VRU2.
Genome annotation databases
GeneID	1499707.
GenomeReviews	Gene locus Bfl507 in contig BX248583_GR.
KEGG	bfl:Bfl507.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VRU2.
OMA	HNQDYID.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL507-MON.
Family and domain databases
HAMAP	MF_01588. [Tree]
InterPro	IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
ProDom	PD003944. DNAligase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00278. HhH1. 3 hits. SM00532. LIGANc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00575. dnlj. 1 hit.
PROSITE	PS50172. BRCT. False negative. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_BLOFL

Accession

Primary (citable) accession number: Q7VRU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)