Reviewed, UniProtKB/Swiss-Prot Q7VRT1 (DAPA_BLOFL)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	Bfl518

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000103095
Regions
	Region	48 – 49	2	Pyruvate binding By similarity
Sites
	Active site	161	1	Schiff-base intermediate with substrate By similarity
	Binding site	106	1	Pyruvate By similarity
	Site	133	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VRT1-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: E487B6F675663EF8	FASTA	292	31,876
	10 20 30 40 50 60 MFTGSIVALI TPMDSKGSID RMGLKKLVDY HVDNGTSAIV SVGTTGEMSG LTHSEHVDVV 70 80 90 100 110 120 MWTIEFSDGR LPVIAGTGSN STSEAILLTN QFNDSAVSAC LSVTPYYNRP SQEGLFQHFR 130 140 150 160 170 180 AIAESTNLPQ ILYNVPIRTG CDLLPITVGR LSTIKNIVGI KEATGNLNRV NQLKRLVNED 190 200 210 220 230 240 FILLSGDDIS AVDFMRLGGH GVISVTANVA PKDMSTLCSF VQKNDFKSAY DINNRLIPVH 250 260 270 280 290 QSLFFDSNPI PVKWACKELG LISYDTLRLP MTSISKEHSV LIRKALEDAD LL

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83204.1.
RefSeq	NP_878798.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHP based on UniProtKB P05640.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VRT1.
Genome annotation databases
GeneID	1499718.
GenomeReviews	Gene locus Bfl518 in contig BX248583_GR.
KEGG	bfl:Bfl518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VRT1.
OMA	VAPRLMH.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL518-MON.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_BLOFL

Accession

Primary (citable) accession number: Q7VRT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)