Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q7VRS6 (URE1_BLOFL)

Last modified November 3, 2009. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha
Gene names
Name: ureC
Ordered Locus Names: Bfl523
OrganismBlochmannia floridanus [Complete proteome] [HAMAP]
Taxonomic identifier203907 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Hide | Top

Protein attributes

Sequence length567 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Catalytic activity

Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953

Cofactor

Binds 2 nickel ions per subunit By similarity.

Pathway

Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953

Subunit structure

Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamylation allows a single lysine to coordinate two nickel ions By similarity.

Sequence similarities

Belongs to the urease family.

Contains 1 urease domain.

Hide | Top

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Nickel
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processurea metabolic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionnickel ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

urease activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 567567Urease subunit alpha HAMAP MF_01953
PRO_0000234134

Regions

Domain129 – 567439Urease

Sites

Active site3201Proton donor By similarity
Metal binding1341Nickel 2 By similarity
Metal binding1361Nickel 2 By similarity
Metal binding2171Nickel 1; via carbamate group By similarity
Metal binding2171Nickel 2; via carbamate group By similarity
Metal binding2461Nickel 1 By similarity
Metal binding2721Nickel 1 By similarity
Metal binding3601Nickel 2 By similarity
Binding site2191Substrate By similarity

Amino acid modifications

Modified residue2171N6-carboxylysine By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q7VRS6-1 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: D100D0F7DD327E09

FASTA56761,658
        10         20         30         40         50         60 
MNMLNKKIYA EMYGPTVGDS VRLADTDLWV KIEKDFTIYG EEVKFGGGKV IRDGMGQGQM 

        70         80         90        100        110        120 
NNKECVDLVL TNAIIIDYWG VIKADIGINN GRIVGIGKSG NPDIQPGVTI YIGPGTEVIS 

       130        140        150        160        170        180 
AEGKIVTAGG IDVHVHFICP QQIEEALSSG MTTLIGGGTG PTTGSNATTC TPGVWFISRM 

       190        200        210        220        230        240 
LQAADALPIN IGFTGKGSSA LPESLIEQIH AGAIGLKVHE DWGATPATID CCLNVADNFD 

       250        260        270        280        290        300 
VQVSIHTDTL NESGFLETTI SAIKNRTIHA YHVEGAGGGH SPDIIRICEL NNVLPSSTNP 

       310        320        330        340        350        360 
TLPYTINTVD EHLDMMMVCH NLNCNLPEDI AFAESRIRRE TIAAEDILHD LGALSMISSD 

       370        380        390        400        410        420 
SQAMGRIGET ILRTWQTAHK MKLQRGSLLG DDQYNDNTRI KRYIAKYTIN PAITHGIAHE 

       430        440        450        460        470        480 
VGSVEIGKLA DLVLWSPVFF GVKPELIVKG GMIVSSVMGD PNASIPTPQP VYYRLMFGSY 

       490        500        510        520        530        540 
DLAKCATRMT FISQSSYDVG VVENLRLNSL IGISKRCRNI KKDHMINNNL KPVMEVDPQT 

       550        560 
YEVRANGELL VCEPVSVVPM SQRYFLF

« Hide

Hide | Top

References

[1]"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes."
Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Hide | Top

Cross-references

Sequence databases

BX248583 Genomic DNA. Translation: CAD83209.1.
RefSeqNP_878803.1.

3D structure databases

HSSPHSSP built from PDB template 1EJX based on UniProtKB P18314.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ7VRS6.

Genome annotation databases

GeneID1499723.
GenomeReviewsGene locus Bfl523 in contig BX248583_GR.
KEGGbfl:Bfl523.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ7VRS6.
OMASHIHFIC.

Enzyme and pathway databases

BioCycCBLO203907:BFL523-MON.

Family and domain databases

HAMAPMF_01953.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR011612. Urease_alpha_N.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR017952. Urease_asu_core.
IPR017950. Urease_asu_CS.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSPR01752. UREASE.
TIGRFAMsTIGR01792. urease_alph. 1 hit.
PROSITEPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameURE1_BLOFL
AccessionPrimary (citable) accession number: Q7VRS6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: October 1, 2003
Last modified: November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents