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Q7VRQ5 (NADK_BLOFL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:Bfl545
OrganismBlochmannia floridanus [Complete proteome] [HAMAP]
Taxonomic identifier203907 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 293293NAD kinase HAMAP-Rule MF_00361
PRO_0000229611

Regions

Nucleotide binding74 – 752NAD By similarity
Nucleotide binding148 – 1492NAD By similarity
Nucleotide binding189 – 1946NAD By similarity

Sites

Active site741Proton acceptor By similarity
Binding site791NAD By similarity
Binding site1761NAD By similarity
Binding site1781NAD By similarity
Binding site2481NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VRQ5 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 9A724E1902B3C0CD

FASTA29332,884
        10         20         30         40         50         60 
MTSSIFRTIG IIGHSSYPKA INTYDVLYRW LYNKGIKVMI EQHASHLLRT HKAIIGNLHD 

        70         80         90        100        110        120 
IGDYADLAIV IGGDGNMLRA ANVLSQYDIK IIGINLGNLG FLTDLNPHSA LIELSKILSG 

       130        140        150        160        170        180 
HFINEKRFLL DIKIQHYNNV TILGTAINEV ILYTNTIKNM IKFELYINNN FTFSSRSDGL 

       190        200        210        220        230        240 
IIATPTGSTA YALSAGGPIL SPSVEGIVLV PICPHTVSSR PIIIDNKSTI SLKFPKITPK 

       250        260        270        280        290 
LTIRCDNQNP IYIDKEKEIF IQRSNHILDL IHPNNYNYFK NLRIKLGWQK NSI 

« Hide

References

[1]"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes."
Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248583 Genomic DNA. Translation: CAD83231.1.
RefSeqNP_878824.1. NC_005061.1.

3D structure databases

ProteinModelPortalQ7VRQ5.
SMRQ7VRQ5. Positions 6-291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203907.Bfl545.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD83231; CAD83231; Bfl545.
GeneID1499745.
KEGGbfl:Bfl545.
PATRIC31964709. VBICanBlo38691_0534.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227221.
KOK00858.
OMATHEMLYH.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycBFLO203907:GHF7-545-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_BLOFL
AccessionPrimary (citable) accession number: Q7VRQ5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families