Q7VRI8 (METE_BLOFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Bfl625

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	769 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 769	769	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098623
Sites
	Metal binding	651	1	Zinc By similarity
	Metal binding	653	1	Zinc By similarity
	Metal binding	737	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VRI8 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 237481A565500CC2	FASTA	769	89,643
	10 20 30 40 50 60 MPVLSHILGF PRIGLYRELK HALEQYWEKK ITENKLLDIG RMLRMRHWKQ QINSGINLIP 70 80 90 100 110 120 IGDFSWYDHV LDMSIMLNNI PTRFHILSQK KNTLNMLFSI ARGDSINNTT ITPSEMKKWF 130 140 150 160 170 180 NTNYHYIVPE FVQNQIFKLN CTQLLAEIDE ALSLNHPIKP VLLGPLTYLW IGKTKETKEF 190 200 210 220 230 240 DRLSLLPSLL LVYKEIFNIL SKKNIQWIQI DEPALVLELP QTWLKAYLHA YDQLYQTKIK 250 260 270 280 290 300 LLLTTYFGKI YHQLPIIKQL KVNGLHIDLT NCSDNDISLL HEQLPKKWIL SAGIINGKNI 310 320 330 340 350 360 WKTNLYDWFL KLNTITHQRS LWIGSSCSLL HSPIDLNIES KLNNNIKNWF AFAIQKCSEI 370 380 390 400 410 420 KLLCDALNSN HNDNSDQQNI LKKYYNTNIQ RLNSNIINNP QVQERCKNII TLDHSRKTKH 430 440 450 460 470 480 ITRMKLQHNR FNLPLYPTTT IGSFPQTSEI RKLRLKFKNK QINEDYYYTH IQQYIKKIIQ 490 500 510 520 530 540 EQEKLDLDIL VHGEPERNDM VEYFGENLKG FVSSQHGWIQ SYGSRCIKPP IIIGDISRPI 550 560 570 580 590 600 PITIPWINYA QSLTNKPVKG ILTGPITIMT WSFAREDIPR HMIALQLALA IRDEVMDLEK 610 620 630 640 650 660 SGIGVIQIDE PALREGLPLK KSEQSYYLKW AINTFKITVS SVKDNTQIHT HMCYSEFNEI 670 680 690 700 710 720 IDDILKLDVD VISIESSRSD LQLLQFIKTA GKKLNEIGPG IYDIHSIHQP SINEIMDKLK 730 740 750 760 KLLQYIPKDR LWINPDCGLK TRSWNEIRES LNNMVIAAKT LRINNGLNN

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248583 Genomic DNA. Translation: CAD83300.1.
RefSeq	NP_878893.1. NC_005061.1.
3D structure databases
ProteinModelPortal	Q7VRI8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VRI8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1499825.
GenomeReviews	Gene locus Bfl625 in contig BX248583_GR.
KEGG	bfl:Bfl625.
PATRIC	31964873. VBICanBlo38691_0606.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q7VRI8.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL625-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BLOFL

Accession

Primary (citable) accession number: Q7VRI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program