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Q7VRA6 (SYL_BLOFL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Bfl313
OrganismBlochmannia floridanus [Complete proteome] [HAMAP]
Taxonomic identifier203907 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia

Protein attributes

Sequence length867 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 867867Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151994

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif625 – 6295"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6281ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VRA6 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: B8A6FB76B78356C9

FASTA867101,320
        10         20         30         40         50         60 
MKKLYFPHQI ERIVQQHWND NQTFSVTEDK NKQKFYCLSM LPYPSGNLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPIGWD AFGLPAEQAA IIHKKNPSDW TYSNIQYMKQ QLKSLGFAYD 

       130        140        150        160        170        180 
WKRELITNDP QYYRWEQWFF IILYEKGLVY RKTTLVNWCP YHNTVLANEQ VINGGCWRCH 

       190        200        210        220        230        240 
TKIQYKKIPQ WFIKITHYAD QLLNGLNQLQ YWPEQVKTMQ RNWIGQSTGT NVIFKILNSN 

       250        260        270        280        290        300 
ITTITVYIAR LDTFMGISYL TISVDHPITL QIAKINPDLA NFISIYNTIS IQLHNKQFIC 

       310        320        330        340        350        360 
HKKKGIFTNL YAVHPITFTK LPIWVANFIT PLEFDGQGAI ASCPAHDQHD WEFAHQYDLP 

       370        380        390        400        410        420 
IKPVIKYADG ELPNITNQAM IEPGILFNSD NFDDLTSHTA INYISKKLID LKIAKTKIFY 

       430        440        450        460        470        480 
HLQDWGVSRQ RYWGVPIPMI KLKNGIIQPV PKSELPVILP EIIYTKNNEN NILSKNFNWT 

       490        500        510        520        530        540 
HTTYKNQDVI RDTDTFDTFM ESSWYYARYT CPHYHDGMLQ SDAANYWLPI DQYIGGIEHA 

       550        560        570        580        590        600 
TMHLMYFRFY HKLMRDIGLI QSNEPAIRLL CQGMILADSF YYISNDGQKN WVSPNKVIST 

       610        620        630        640        650        660 
RDKMGHIIKS IDADGNNVIY AGLCKMSKSK NNGIDPNAMI QKYGADAVRF FIMFAAPAHS 

       670        680        690        700        710        720 
TLEWKESGIE GALRFLKRLW NITYQHIQNG LIHQLNMYKL ENKHKIIRQK VHETIIKVTD 

       730        740        750        760        770        780 
DIDRRQSFNT ALAAIMKLFN DIQDIFPINN TQDRSVLHEA LSIIVKLLYP FTPHISYILW 

       790        800        810        820        830        840 
KELGYTNTID DTTWPTPDLQ AIQTQEILII VQINGKKKKK IFVPINSDKN TIHEIAKQAI 

       850        860 
YQDKNLESKY VHKIIYIPNK IINFITK 

« Hide

References

[1]"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes."
Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248583 Genomic DNA. Translation: CAD83383.1.
RefSeqNP_878608.1. NC_005061.1.

3D structure databases

ProteinModelPortalQ7VRA6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203907.Bfl313.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD83383; CAD83383; Bfl313.
GeneID1499513.
KEGGbfl:Bfl313.
PATRIC31964227. VBICanBlo38691_0307.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBFLO203907:GHF7-313-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BLOFL
AccessionPrimary (citable) accession number: Q7VRA6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries