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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Blochmannia floridanus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei628 – 6281ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBFLO203907:GHF7-313-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Bfl313
OrganismiBlochmannia floridanus
Taxonomic identifieri203907 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
ProteomesiUP000002192 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 867867Leucine--tRNA ligasePRO_0000151994Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203907.Bfl313.

Structurei

3D structure databases

ProteinModelPortaliQ7VRA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi625 – 6295"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VRA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLYFPHQI ERIVQQHWND NQTFSVTEDK NKQKFYCLSM LPYPSGNLHM
60 70 80 90 100
GHVRNYTIGD VISRYQRMLG KNVLQPIGWD AFGLPAEQAA IIHKKNPSDW
110 120 130 140 150
TYSNIQYMKQ QLKSLGFAYD WKRELITNDP QYYRWEQWFF IILYEKGLVY
160 170 180 190 200
RKTTLVNWCP YHNTVLANEQ VINGGCWRCH TKIQYKKIPQ WFIKITHYAD
210 220 230 240 250
QLLNGLNQLQ YWPEQVKTMQ RNWIGQSTGT NVIFKILNSN ITTITVYIAR
260 270 280 290 300
LDTFMGISYL TISVDHPITL QIAKINPDLA NFISIYNTIS IQLHNKQFIC
310 320 330 340 350
HKKKGIFTNL YAVHPITFTK LPIWVANFIT PLEFDGQGAI ASCPAHDQHD
360 370 380 390 400
WEFAHQYDLP IKPVIKYADG ELPNITNQAM IEPGILFNSD NFDDLTSHTA
410 420 430 440 450
INYISKKLID LKIAKTKIFY HLQDWGVSRQ RYWGVPIPMI KLKNGIIQPV
460 470 480 490 500
PKSELPVILP EIIYTKNNEN NILSKNFNWT HTTYKNQDVI RDTDTFDTFM
510 520 530 540 550
ESSWYYARYT CPHYHDGMLQ SDAANYWLPI DQYIGGIEHA TMHLMYFRFY
560 570 580 590 600
HKLMRDIGLI QSNEPAIRLL CQGMILADSF YYISNDGQKN WVSPNKVIST
610 620 630 640 650
RDKMGHIIKS IDADGNNVIY AGLCKMSKSK NNGIDPNAMI QKYGADAVRF
660 670 680 690 700
FIMFAAPAHS TLEWKESGIE GALRFLKRLW NITYQHIQNG LIHQLNMYKL
710 720 730 740 750
ENKHKIIRQK VHETIIKVTD DIDRRQSFNT ALAAIMKLFN DIQDIFPINN
760 770 780 790 800
TQDRSVLHEA LSIIVKLLYP FTPHISYILW KELGYTNTID DTTWPTPDLQ
810 820 830 840 850
AIQTQEILII VQINGKKKKK IFVPINSDKN TIHEIAKQAI YQDKNLESKY
860
VHKIIYIPNK IINFITK
Length:867
Mass (Da):101,320
Last modified:September 30, 2003 - v1
Checksum:iB8A6FB76B78356C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248583 Genomic DNA. Translation: CAD83383.1.
RefSeqiNP_878608.1. NC_005061.1.
WP_011126591.1. NC_005061.1.

Genome annotation databases

EnsemblBacteriaiCAD83383; CAD83383; Bfl313.
KEGGibfl:Bfl313.
PATRICi31964227. VBICanBlo38691_0307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248583 Genomic DNA. Translation: CAD83383.1.
RefSeqiNP_878608.1. NC_005061.1.
WP_011126591.1. NC_005061.1.

3D structure databases

ProteinModelPortaliQ7VRA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203907.Bfl313.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD83383; CAD83383; Bfl313.
KEGGibfl:Bfl313.
PATRICi31964227. VBICanBlo38691_0307.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBFLO203907:GHF7-313-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYL_BLOFL
AccessioniPrimary (citable) accession number: Q7VRA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2004
Last sequence update: September 30, 2003
Last modified: March 31, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.