Reviewed, UniProtKB/Swiss-Prot Q7VR67 (SYFA_BLOFL)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Bfl355

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000126683
Sites
	Metal binding	254	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VR67-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 232A5BEB7B156CCC	FASTA	335	39,057
	10 20 30 40 50 60 MPKKLLINLV KQAKLSLEQS NTIQDVESIR IQFLGKHGYI NQQLKKINSL NWDNKSKIGT 70 80 90 100 110 120 VINQVKSDIH QLFLERKKLI TLKAIEKSLN TEKLDITLPG RLSETGTIHP ITNTINRIRL 130 140 150 160 170 180 FFNHLGFKEI YGPEIENSYF NFDSLNIPNN HPSRDQHDTF WFDTNKLLRT HTSGIQIRAM 190 200 210 220 230 240 KHIPMPIQII SIGKVYRKDY NKEHTPMFHQ IEGMMISSNI NLTNLKKILY DFLYNFFGKN 250 260 270 280 290 300 VILRFRASYF PFTEPSAEID ILNLNINNKK QPKKWIELLG CGMIHPTVLN NGGIDSKKFS 310 320 330 GFAFGIGIER LTMLLYHMTD IRVFFENDLQ FLDQF

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83422.1.
RefSeq	NP_878647.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VR67.
Genome annotation databases
GeneID	1499555.
GenomeReviews	Gene locus Bfl355 in contig BX248583_GR.
KEGG	bfl:Bfl355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VR67.
OMA	FRASYFP.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL355-MON.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BLOFL

Accession

Primary (citable) accession number: Q7VR67

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)