Reviewed, UniProtKB/Swiss-Prot Q7VR53 (PDXH_BLOFL)

Names and origin

Protein names

Recommended name:
    Pyridoxine/pyridoxamine 5'-phosphate oxidase
    EC=1.4.3.5
Alternative name(s):
    PNP/PMP oxidase
      Short name=PNPOx
    Pyridoxal 5'-phosphate synthase

Gene names

Name:	pdxH
Ordered Locus Names:	Bfl370

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP) By similarity.
Catalytic activity	Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2. HAMAP MF_01629 Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2. HAMAP MF_01629
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1. HAMAP MF_01629 Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the pyridoxamine 5'-phosphate oxidase family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: HAMAP pyridoxamine-phosphate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Pyridoxine/pyridoxamine 5'-phosphate oxidase HAMAP MF_01629		PRO_0000167687
Regions
	Nucleotide binding	78 – 79	2	FMN By similarity
	Nucleotide binding	142 – 143	2	FMN By similarity
	Region	11 – 14	4	Substrate binding By similarity
	Region	193 – 195	3	Substrate binding By similarity
Sites
	Binding site	63	1	FMN By similarity
	Binding site	66	1	FMN; via amide nitrogen By similarity
	Binding site	68	1	Substrate By similarity
	Binding site	85	1	FMN By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	129	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VR53-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 4288EFDB7B9116EB	FASTA	214	25,820
	10 20 30 40 50 60 MLELSKIHNL RREYISKQFR RSNLTKNPMH LFSKWLYEAY CQIPDPNAMC LSTVDHTGQP 70 80 90 100 110 120 FQRLVLLKYF NDKTIVFFTH LNSRKAIHIN NNPKISLCFP WNIINRQIII TGSVYKISKK 130 140 150 160 170 180 EAQKYFYTRP KNNQISTWAS KQSTIISSKK VLKNKFLKLK KKYFQKSVPF PHFWVGYKIN 190 200 210 INSMEFWQGG IYRLHDRFLY KKNKKKWYIN RLSP

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83436.1.
RefSeq	NP_878661.1.
3D structure databases
HSSP	HSSP built from PDB template 1JNW based on UniProtKB P28225.
ModBase	Search...
Genome annotation databases
GeneID	1499570.
GenomeReviews	Gene locus Bfl370 in contig BX248583_GR.
KEGG	bfl:Bfl370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VR53.
OMA	Q7VR53. FTFFTNY.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL370-MON.
Family and domain databases
HAMAP	MF_01629. [Tree]
InterPro	IPR011576. PNPOx_rel_FMN_bd_core. IPR000659. Pyridoxamine_oxidase. IPR019740. Pyridoxamine_oxidase_CS. IPR019576. Pyridoxamine_oxidase_dimer_C. IPR012349. Split_barrel_FMN_bd. [Graphical view]
Gene3D	G3DSA:2.30.110.10. PNPOx_FMN_bd. 1 hit.
PANTHER	PTHR10851. Pyridox_oxidase. 1 hit.
Pfam	PF10590. PNPOx_C. 1 hit. PF01243. Pyridox_oxidase. 1 hit. [Graphical view]
ProDom	PD006312. Pyridox_oxidase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00558. pdxH. 1 hit.
PROSITE	PS01064. PYRIDOX_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDXH_BLOFL

Accession

Primary (citable) accession number: Q7VR53

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)