Reviewed, UniProtKB/Swiss-Prot Q7VR52 (SYY_BLOFL)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	Bfl371

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Tyrosyl-tRNA synthetase HAMAP MF_02006		PRO_0000234684
Regions
	Domain	354 – 411	58	S4 RNA-binding
	Motif	41 – 50	10	"HIGH" region HAMAP MF_02006
	Motif	235 – 239	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	36	1	Tyrosine By similarity
	Binding site	175	1	Tyrosine By similarity
	Binding site	179	1	Tyrosine By similarity
	Binding site	238	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VR52-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: B5B397E9DAC5D4D3	FASTA	422	48,952
	10 20 30 40 50 60 MIDNIIDYLY ERGSIAQITD RKKLNAVLNS QPITLYCGFD PTADSLHIGH LAPLLCLKRF 70 80 90 100 110 120 QKAGHRPLIL LGGATGLIGD PSFKDSERKL QSFNTTQKWI KKIKLQIALF IDCNSNNASQ 130 140 150 160 170 180 AHIIDNYSWF SSMNILTFLR DIGKFFSINK MINKDIIKKR LKNDNYGISY TEFSYNLMQS 190 200 210 220 230 240 YDFAHIYKHY DAILQIGGSD QWGNIISGID LIKRMYKKNA YGLTVPLLTN FNNIKFGKTE 250 260 270 280 290 300 KNTIWLDPEK TSPYQFYQYW INIDDKSAYH FLKIFTDISV QDINSFKNRD HSYSQAQYIL 310 320 330 340 350 360 AENMTQLVHG KRGLNIAQRI SQNLFSYNIL KLTLNDFHQL IQDGIPNIIL ETNTSLQEAL 370 380 390 400 410 420 TKSKLATSRS QARYFIKSNA ITINAHKQSK IEYIFQDSDR IYNLYTLLKR GKKNYCLIQW NI

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248583 Genomic DNA. Translation: CAD83437.1.
RefSeq	NP_878662.1.
3D structure databases
SMR	Q7VR52. Positions 4-319, 5-420.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VR52.
Genome annotation databases
GeneID	1499571.
GenomeReviews	Gene locus Bfl371 in contig BX248583_GR.
KEGG	bfl:Bfl371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	TFYIGFD.
PhylomeDB	Q7VR52.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL371-MONOMER.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_BLOFL

Accession

Primary (citable) accession number: Q7VR52

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)