Reviewed, UniProtKB/Swiss-Prot Q7VR50 (FUMC_BLOFL)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	Bfl373

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161264
Regions
	Region	113 – 116	4	B site By similarity
	Region	123 – 125	3	Substrate binding By similarity
Sites
	Binding site	85	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VR50-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: CDEC7863B7D10F2D	FASTA	448	49,649
	10 20 30 40 50 60 MICNRLWGDQ TESSLKFFNI STEKMPWELI KALAQIKRVS AQVNYDLKLL DYERSQAIIA 70 80 90 100 110 120 AVDEILSGNH KNEFPLSVWQ TGSGTQSNMN MNEVLANRAN ELLRKNQINI VVHPNDHVNK 130 140 150 160 170 180 SQSSNDVFPS AMHIAAVVNL KTKLIPVIKL LQKTFLKKSI EFRNIIKIGR THLQDAIPLT 190 200 210 220 230 240 LGQEISGWDF MLKNNTNHIQ STILDLSALA LGGTAVGTGF SAHVEYAERV VLGLSKLIHH 250 260 270 280 290 300 SFFSAPNKFE SLSTCDAIVY SHGTLKGLAI SMMKIANDIR LLSSGPQCGL GELIIPANEP 310 320 330 340 350 360 GSSIMPGKVN PTQCESMTMS CCQVMGNDLS ISLGGSSGQL QLNTYRPLII YNFLQSIRLL 370 380 390 400 410 420 TDSIKNFHDY CIVGIRPKFK RIEKLLNKSL MLVTALSSHI GYDKSAQIAQ TAYLNGITLK 430 440 AASIQSGYVT EKQFDDWVCP ENMIYPDM

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248583 Genomic DNA. Translation: CAD83439.1.
RefSeq	NP_878664.1.
3D structure databases
SMR	Q7VR50. Positions 4-444.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VR50.
Genome annotation databases
GeneID	1499573.
GenomeReviews	Gene locus Bfl373 in contig BX248583_GR.
KEGG	bfl:Bfl373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0114.
HOGENOM	HBG284369.
OMA	RIEKDTM.
PhylomeDB	Q7VR50.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL373-MONOMER.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_BLOFL

Accession

Primary (citable) accession number: Q7VR50

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)