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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Blochmannia floridanus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171SubstrateUniRule annotation1
Active sitei172Proton donor/acceptorUniRule annotation1
Active sitei302UniRule annotation1
Binding sitei303SubstrateUniRule annotation1
Sitei315Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:Bfl373
OrganismiBlochmannia floridanus
Taxonomic identifieri203907 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
Proteomesi
  • UP000002192 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001612641 – 448Fumarate hydratase class IIAdd BLAST448

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi203907.Bfl373.

Structurei

3D structure databases

ProteinModelPortaliQ7VR50.
SMRiQ7VR50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni83 – 85Substrate bindingUniRule annotation3
Regioni113 – 116Substrate binding (B site)UniRule annotation4
Regioni123 – 125Substrate bindingUniRule annotation3
Regioni308 – 310Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7VR50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MICNRLWGDQ TESSLKFFNI STEKMPWELI KALAQIKRVS AQVNYDLKLL
60 70 80 90 100
DYERSQAIIA AVDEILSGNH KNEFPLSVWQ TGSGTQSNMN MNEVLANRAN
110 120 130 140 150
ELLRKNQINI VVHPNDHVNK SQSSNDVFPS AMHIAAVVNL KTKLIPVIKL
160 170 180 190 200
LQKTFLKKSI EFRNIIKIGR THLQDAIPLT LGQEISGWDF MLKNNTNHIQ
210 220 230 240 250
STILDLSALA LGGTAVGTGF SAHVEYAERV VLGLSKLIHH SFFSAPNKFE
260 270 280 290 300
SLSTCDAIVY SHGTLKGLAI SMMKIANDIR LLSSGPQCGL GELIIPANEP
310 320 330 340 350
GSSIMPGKVN PTQCESMTMS CCQVMGNDLS ISLGGSSGQL QLNTYRPLII
360 370 380 390 400
YNFLQSIRLL TDSIKNFHDY CIVGIRPKFK RIEKLLNKSL MLVTALSSHI
410 420 430 440
GYDKSAQIAQ TAYLNGITLK AASIQSGYVT EKQFDDWVCP ENMIYPDM
Length:448
Mass (Da):49,649
Last modified:October 1, 2003 - v1
Checksum:iCDEC7863B7D10F2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248583 Genomic DNA. Translation: CAD83439.1.
RefSeqiWP_011126647.1. NC_005061.1.

Genome annotation databases

EnsemblBacteriaiCAD83439; CAD83439; Bfl373.
KEGGibfl:Bfl373.

Similar proteinsi

Entry informationi

Entry nameiFUMC_BLOFL
AccessioniPrimary (citable) accession number: Q7VR50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2003
Last modified: August 30, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families