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Protein

Imidazole glycerol phosphate synthase subunit HisH

Gene

hisH

Organism
Blochmannia floridanus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR.UniRule annotation

Catalytic activityi

5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O.UniRule annotation

Pathwayi: L-histidine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamideisomerase (hisA), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei77 – 771NucleophileUniRule annotation
Active sitei178 – 1781UniRule annotation
Active sitei180 – 1801UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciBFLO203907:GHF7-466-MONOMER.
UniPathwayiUPA00031; UER00010.

Names & Taxonomyi

Protein namesi
Recommended name:
Imidazole glycerol phosphate synthase subunit HisHUniRule annotation (EC:2.4.2.-UniRule annotation)
Alternative name(s):
IGP synthase glutamine amidotransferase subunitUniRule annotation
IGP synthase subunit HisHUniRule annotation
ImGP synthase subunit HisHUniRule annotation
Short name:
IGPS subunit HisHUniRule annotation
Gene namesi
Name:hisHUniRule annotation
Ordered Locus Names:Bfl466
OrganismiBlochmannia floridanus
Taxonomic identifieri203907 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
Proteomesi
  • UP000002192 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Imidazole glycerol phosphate synthase subunit HisHPRO_0000152363Add
BLAST

Interactioni

Subunit structurei

Heterodimer of HisH and HisF.UniRule annotation

Protein-protein interaction databases

STRINGi203907.Bfl466.

Structurei

3D structure databases

ProteinModelPortaliQ7VQW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 196195Glutamine amidotransferase type-1UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108UJE. Bacteria.
COG0118. LUCA.
HOGENOMiHOG000025030.
KOiK02501.
OMAiSTIAQCH.
OrthoDBiEOG69KV05.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00278. HisH.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010139. Imidazole-glycPsynth_HisH.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
PIRSFiPIRSF000495. Amidotransf_hisH. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01855. IMP_synth_hisH. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VQW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIIIINTNC SNLSSVKIML NKLGCNTIIT DDPDLIIQSD KILLPGVGTA
60 70 80 90 100
NTAMKQLKKK NLIQLIKKCT QPTLGICLGM QLFGSFSNEN THTNTLNIIN
110 120 130 140 150
VPVKHMYSPT LPIPHMGWNN ITILKKHDLL NGINNNHYFY FAHSYCMELN
160 170 180 190
YTTIAYTNYG QPFSSVIVYK NFFGVQFHPE KSSFSGEQLI KNFLEI
Length:196
Mass (Da):22,177
Last modified:October 1, 2003 - v1
Checksum:iF6521F5982608187
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248583 Genomic DNA. Translation: CAD83525.1.
RefSeqiWP_011126732.1. NC_005061.1.

Genome annotation databases

EnsemblBacteriaiCAD83525; CAD83525; Bfl466.
KEGGibfl:Bfl466.
PATRICi31964543. VBICanBlo38691_0454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248583 Genomic DNA. Translation: CAD83525.1.
RefSeqiWP_011126732.1. NC_005061.1.

3D structure databases

ProteinModelPortaliQ7VQW7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203907.Bfl466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD83525; CAD83525; Bfl466.
KEGGibfl:Bfl466.
PATRICi31964543. VBICanBlo38691_0454.

Phylogenomic databases

eggNOGiENOG4108UJE. Bacteria.
COG0118. LUCA.
HOGENOMiHOG000025030.
KOiK02501.
OMAiSTIAQCH.
OrthoDBiEOG69KV05.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00010.
BioCyciBFLO203907:GHF7-466-MONOMER.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00278. HisH.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010139. Imidazole-glycPsynth_HisH.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
PIRSFiPIRSF000495. Amidotransf_hisH. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01855. IMP_synth_hisH. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiHIS5_BLOFL
AccessioniPrimary (citable) accession number: Q7VQW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: October 1, 2003
Last modified: November 11, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.