Reviewed, UniProtKB/Swiss-Prot Q7VQT0 (AMPA_BLOFL)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Bfl035

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165735
Sites
	Active site	286	1	Potential
	Active site	360	1	Potential
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	279	1	Manganese 1 By similarity
	Metal binding	279	1	Manganese 2 By similarity
	Metal binding	297	1	Manganese 2 By similarity
	Metal binding	356	1	Manganese 1 By similarity
	Metal binding	358	1	Manganese 1 By similarity
	Metal binding	358	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VQT0-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: E45634F0CC1DA3DD	FASTA	504	56,345
	10 20 30 40 50 60 MPIKFDVTQE HIESYPNFCI VTGVFNDSYL FPSTIQIDKM SQGYISSLLK RNNFNGSINQ 70 80 90 100 110 120 HLLLYDAPYF FNKLILLIGC GKKNEFKSQS YNTVIHYIIN FCKKSSITKI LLLLSELNID 130 140 150 160 170 180 GYDNYWKIRN SITLCNNECY IFNKFKNIND VTKKNITYEI ISYIDKLDNN FQYCKQAVKD 190 200 210 220 230 240 GLAIAKGLNI AKNLGNMPSN YCTPNYLSNK VQELSNYNTD LDIEIINELQ LKTIGMHAYL 250 260 270 280 290 300 AVGSGSIYPA VMPIIKYQKN PKGPNTNPII LIGKGVTFDS GGISIKPSDK MDEMKYDMCG 310 320 330 340 350 360 AAAVYAIMSI VSELQLPLNI IGILAVSENM ISSKSLKPGD ILTTLSGQTI EILNTDAEGR 370 380 390 400 410 420 LLLCDVLTYV ERYNPEIVID IATLTGACVI ALGNHYSGLM SNDNNLSNNL IFASKQTRDY 430 440 450 460 470 480 IWRLPLDENF EKQLESSCAD IANVGGRSGG AITAACFLKK FAHKYKWMHL DIAGSAWISK 490 500 NCVKSSTGRP VELLTQFLIN ISKI

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83563.1.
RefSeq	NP_878350.1.
3D structure databases
HSSP	HSSP built from PDB template 1GYT based on UniProtKB P11648.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VQT0.
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	1499235.
GenomeReviews	Gene locus Bfl035 in contig BX248583_GR.
KEGG	bfl:Bfl035.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VQT0.
OMA	LGHHISG.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL035-MON.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BLOFL

Accession

Primary (citable) accession number: Q7VQT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)