Reviewed, UniProtKB/Swiss-Prot Q7VQP1 (PURA_BLOFL)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	Bfl083

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000095162
Regions
	Nucleotide binding	13 – 19	7	GTP Potential
Sites
	Active site	147	1	By similarity
	Active site	154	1	By similarity
	Metal binding	14	1	Magnesium By similarity
	Metal binding	41	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VQP1-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 1B2BB5DC22A91251	FASTA	438	48,856
	10 20 30 40 50 60 MIQNIVIIGT QWGDEGKGKI IDFLTPQVQY VVRYQGGHNA GHTIVVNHKK VTLHLIPSGI 70 80 90 100 110 120 LHDNIINIIA GGVVLSPVIL VKEIQKLKKI NVLVDDRIFI SESCSLILPY HIAIDLAREK 130 140 150 160 170 180 ISTTVITNNN VIIGTTGCGI GPAYEDKVAR RALRVYDLYN LKNFENKLKN IADFYNFQLV 190 200 210 220 230 240 QYYHAEPIDY HVVMDEIITV SDILIKKVID VPELLLNARK RGDKIIFEGA QGSLLDIDHG 250 260 270 280 290 300 TYPYVTSSHT TSGGAIVGIG VGPKYIDYVL GIMKAYSTRV GNGPFPTELS GDIAEWLCTR 310 320 330 340 350 360 GQEFGSTTGR RRRTGWFDAV SVRHAIKISS INSACLTKID ILDGLKYVKI CVAYQSFNGE 370 380 390 400 410 420 IIYNFPYSLE NLKNITPVYE VLPGWSGRTT GIKKFNQLPL EAKQYIKRIE EVIGIPIDII 430 STGPDRLMNI IVRNPLNF

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83606.1.
RefSeq	NP_878393.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VQP1.
Genome annotation databases
GeneID	1499283.
GenomeReviews	Gene locus Bfl083 in contig BX248583_GR.
KEGG	bfl:Bfl083.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VQP1.
OMA	YVLGIIK.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL083-MON.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_BLOFL

Accession

Primary (citable) accession number: Q7VQP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)