Reviewed, UniProtKB/Swiss-Prot Q7VQL0 (SYI_BLOFL)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	Bfl118

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_02002
Subunit structure	Monomer By similarity. HAMAP MF_02002
Subcellular location	Cytoplasm By similarity HAMAP MF_02002.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 953	953	Isoleucyl-tRNA synthetase HAMAP MF_02002		PRO_0000098355
Regions
	Motif	58 – 68	11	"HIGH" region HAMAP MF_02002
	Motif	614 – 618	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	916	1	Zinc By similarity
	Metal binding	919	1	Zinc By similarity
	Metal binding	936	1	Zinc By similarity
	Metal binding	939	1	Zinc By similarity
	Binding site	573	1	Aminoacyl-adenylate By similarity
	Binding site	617	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VQL0-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: E4991E114C404932	FASTA	953	111,929
	10 20 30 40 50 60 MADYKSTLNL PYTQFPMRGN LPIIEIKILE RWNRDNLYEI IRRKKNEKKL FLLHDGPPYA 70 80 90 100 110 120 NGFIHLGHAV NKILKDIIIK FKGLSGYDAP YIPGWDCHGL PIELQVEKLI KKVNMDIDIN 130 140 150 160 170 180 SQEFRNYCRE YVKKQIEIQK KDFIRLGILG EWKNPYLTMD YKTEANIIRT LGKIISNGYF 190 200 210 220 230 240 YKGIKPVYWC FQCHSALANS EVEYNDYHYS NAVDVGFSIV ENVSINKIFN INCYIENIEL 250 260 270 280 290 300 VIWTTTIWTL PANQAISIHP DYIYQLVKIL DNEKYLIIAA NLVNMFMNRI KCTMWQVLGE 310 320 330 340 350 360 VLGSKLDRLT ARHPFMNFNV PLVLSKHIKL DSGTGLVHIA PDHGPDDYLI SKKYKFKNRD 370 380 390 400 410 420 SLIDSNGYYL SNSHNRLCGL HIFNANEIII DLLYKSKNFL YFNANYQHSY PYCWRHKIPL 430 440 450 460 470 480 IFRTTSQWFV NMDHNNLRDK LLRTLQQVRW IPDSGYSSMQ SMIVNRPDWC LSRQRVWGIP 490 500 510 520 530 540 IPVFVHKKTE VLHPNTCIFI EQVAQLVEKY GIQIWWDLKN EDIILNKAES MNYQKIYDTL 550 560 570 580 590 600 DVWFDSGSTH DSVILDRFNS KLKSKLQIDL YLEGVDQYRG WFMSSLIIAV AIKGYAPYKQ 610 620 630 640 650 660 VLSHGFTIDD KGNKMSKSLG NIIRPLDIVN KFGSDILRLW VASSDYSKDM VISDDVLKNV 670 680 690 700 710 720 TDIYRRIRNT IRFFLANIND FDPEKDLVQS NRMVALDQWA INHTLSVQVK IISNYEQYKF 730 740 750 760 770 780 HNVIRYIMKF CSIEMGSFYL DVVKDRLYTL NKDSLARRSC QTALYHIIES MVRWIAPILS 790 800 810 820 830 840 FTADEIWKYI PGNRSKYVFT EEWYDRLFKI DENQIVNSNY WNFFLNIRNK VNKVIEQERV 850 860 870 880 890 900 NGIIKGSLEA DVILYVTPIL KKKLRILKNE LAFGLIVSSV MVLSIDDVDF NTIKENHEEN 910 920 930 940 950 SDELKVVLKK SHGIKCLRCW NYTLSMSKNE NYLNICSRCV HNITGLGEDR RFF

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248583 Genomic DNA. Translation: CAD83639.1.
RefSeq	NP_878425.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VQL0.
Genome annotation databases
GeneID	1499318.
GenomeReviews	Gene locus Bfl118 in contig BX248583_GR.
KEGG	bfl:Bfl118.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HBG577712.
OMA	KQVLTHG.
PhylomeDB	Q7VQL0.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL118-MONOMER.
Family and domain databases
HAMAP	MF_02002. Ile_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_Ia_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_BLOFL

Accession

Primary (citable) accession number: Q7VQL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)