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Q7VQL0

- SYI_BLOFL

UniProt

Q7VQL0 - SYI_BLOFL

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Blochmannia floridanus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei573 – 5731Aminoacyl-adenylateUniRule annotation
    Binding sitei617 – 6171ATPUniRule annotation
    Metal bindingi916 – 9161ZincUniRule annotation
    Metal bindingi919 – 9191ZincUniRule annotation
    Metal bindingi936 – 9361ZincUniRule annotation
    Metal bindingi939 – 9391ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBFLO203907:GHF7-118-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Bfl118
    OrganismiBlochmannia floridanus
    Taxonomic identifieri203907 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
    ProteomesiUP000002192: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Isoleucine--tRNA ligasePRO_0000098355Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi203907.Bfl118.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7VQL0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi614 – 6185"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7VQL0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADYKSTLNL PYTQFPMRGN LPIIEIKILE RWNRDNLYEI IRRKKNEKKL    50
    FLLHDGPPYA NGFIHLGHAV NKILKDIIIK FKGLSGYDAP YIPGWDCHGL 100
    PIELQVEKLI KKVNMDIDIN SQEFRNYCRE YVKKQIEIQK KDFIRLGILG 150
    EWKNPYLTMD YKTEANIIRT LGKIISNGYF YKGIKPVYWC FQCHSALANS 200
    EVEYNDYHYS NAVDVGFSIV ENVSINKIFN INCYIENIEL VIWTTTIWTL 250
    PANQAISIHP DYIYQLVKIL DNEKYLIIAA NLVNMFMNRI KCTMWQVLGE 300
    VLGSKLDRLT ARHPFMNFNV PLVLSKHIKL DSGTGLVHIA PDHGPDDYLI 350
    SKKYKFKNRD SLIDSNGYYL SNSHNRLCGL HIFNANEIII DLLYKSKNFL 400
    YFNANYQHSY PYCWRHKIPL IFRTTSQWFV NMDHNNLRDK LLRTLQQVRW 450
    IPDSGYSSMQ SMIVNRPDWC LSRQRVWGIP IPVFVHKKTE VLHPNTCIFI 500
    EQVAQLVEKY GIQIWWDLKN EDIILNKAES MNYQKIYDTL DVWFDSGSTH 550
    DSVILDRFNS KLKSKLQIDL YLEGVDQYRG WFMSSLIIAV AIKGYAPYKQ 600
    VLSHGFTIDD KGNKMSKSLG NIIRPLDIVN KFGSDILRLW VASSDYSKDM 650
    VISDDVLKNV TDIYRRIRNT IRFFLANIND FDPEKDLVQS NRMVALDQWA 700
    INHTLSVQVK IISNYEQYKF HNVIRYIMKF CSIEMGSFYL DVVKDRLYTL 750
    NKDSLARRSC QTALYHIIES MVRWIAPILS FTADEIWKYI PGNRSKYVFT 800
    EEWYDRLFKI DENQIVNSNY WNFFLNIRNK VNKVIEQERV NGIIKGSLEA 850
    DVILYVTPIL KKKLRILKNE LAFGLIVSSV MVLSIDDVDF NTIKENHEEN 900
    SDELKVVLKK SHGIKCLRCW NYTLSMSKNE NYLNICSRCV HNITGLGEDR 950
    RFF 953
    Length:953
    Mass (Da):111,929
    Last modified:October 1, 2003 - v1
    Checksum:iE4991E114C404932
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248583 Genomic DNA. Translation: CAD83639.1.
    RefSeqiNP_878425.1. NC_005061.1.
    WP_011126408.1. NC_005061.1.

    Genome annotation databases

    EnsemblBacteriaiCAD83639; CAD83639; Bfl118.
    GeneIDi1499318.
    KEGGibfl:Bfl118.
    PATRICi31963831. VBICanBlo38691_0118.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248583 Genomic DNA. Translation: CAD83639.1 .
    RefSeqi NP_878425.1. NC_005061.1.
    WP_011126408.1. NC_005061.1.

    3D structure databases

    ProteinModelPortali Q7VQL0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203907.Bfl118.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD83639 ; CAD83639 ; Bfl118 .
    GeneIDi 1499318.
    KEGGi bfl:Bfl118.
    PATRICi 31963831. VBICanBlo38691_0118.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BFLO203907:GHF7-118-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiSYI_BLOFL
    AccessioniPrimary (citable) accession number: Q7VQL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3