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Q7VQL0

- SYI_BLOFL

UniProt

Q7VQL0 - SYI_BLOFL

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Bfl118
Organism
Blochmannia floridanus
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei573 – 5731Aminoacyl-adenylate By similarity
Binding sitei617 – 6171ATP By similarity
Metal bindingi916 – 9161Zinc By similarity
Metal bindingi919 – 9191Zinc By similarity
Metal bindingi936 – 9361Zinc By similarity
Metal bindingi939 – 9391Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBFLO203907:GHF7-118-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Bfl118
OrganismiBlochmannia floridanus
Taxonomic identifieri203907 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
ProteomesiUP000002192: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Isoleucine--tRNA ligaseUniRule annotationPRO_0000098355Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi203907.Bfl118.

Structurei

3D structure databases

ProteinModelPortaliQ7VQL0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi614 – 6185"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VQL0-1 [UniParc]FASTAAdd to Basket

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MADYKSTLNL PYTQFPMRGN LPIIEIKILE RWNRDNLYEI IRRKKNEKKL    50
FLLHDGPPYA NGFIHLGHAV NKILKDIIIK FKGLSGYDAP YIPGWDCHGL 100
PIELQVEKLI KKVNMDIDIN SQEFRNYCRE YVKKQIEIQK KDFIRLGILG 150
EWKNPYLTMD YKTEANIIRT LGKIISNGYF YKGIKPVYWC FQCHSALANS 200
EVEYNDYHYS NAVDVGFSIV ENVSINKIFN INCYIENIEL VIWTTTIWTL 250
PANQAISIHP DYIYQLVKIL DNEKYLIIAA NLVNMFMNRI KCTMWQVLGE 300
VLGSKLDRLT ARHPFMNFNV PLVLSKHIKL DSGTGLVHIA PDHGPDDYLI 350
SKKYKFKNRD SLIDSNGYYL SNSHNRLCGL HIFNANEIII DLLYKSKNFL 400
YFNANYQHSY PYCWRHKIPL IFRTTSQWFV NMDHNNLRDK LLRTLQQVRW 450
IPDSGYSSMQ SMIVNRPDWC LSRQRVWGIP IPVFVHKKTE VLHPNTCIFI 500
EQVAQLVEKY GIQIWWDLKN EDIILNKAES MNYQKIYDTL DVWFDSGSTH 550
DSVILDRFNS KLKSKLQIDL YLEGVDQYRG WFMSSLIIAV AIKGYAPYKQ 600
VLSHGFTIDD KGNKMSKSLG NIIRPLDIVN KFGSDILRLW VASSDYSKDM 650
VISDDVLKNV TDIYRRIRNT IRFFLANIND FDPEKDLVQS NRMVALDQWA 700
INHTLSVQVK IISNYEQYKF HNVIRYIMKF CSIEMGSFYL DVVKDRLYTL 750
NKDSLARRSC QTALYHIIES MVRWIAPILS FTADEIWKYI PGNRSKYVFT 800
EEWYDRLFKI DENQIVNSNY WNFFLNIRNK VNKVIEQERV NGIIKGSLEA 850
DVILYVTPIL KKKLRILKNE LAFGLIVSSV MVLSIDDVDF NTIKENHEEN 900
SDELKVVLKK SHGIKCLRCW NYTLSMSKNE NYLNICSRCV HNITGLGEDR 950
RFF 953
Length:953
Mass (Da):111,929
Last modified:October 1, 2003 - v1
Checksum:iE4991E114C404932
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248583 Genomic DNA. Translation: CAD83639.1.
RefSeqiNP_878425.1. NC_005061.1.
WP_011126408.1. NC_005061.1.

Genome annotation databases

EnsemblBacteriaiCAD83639; CAD83639; Bfl118.
GeneIDi1499318.
KEGGibfl:Bfl118.
PATRICi31963831. VBICanBlo38691_0118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248583 Genomic DNA. Translation: CAD83639.1 .
RefSeqi NP_878425.1. NC_005061.1.
WP_011126408.1. NC_005061.1.

3D structure databases

ProteinModelPortali Q7VQL0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203907.Bfl118.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD83639 ; CAD83639 ; Bfl118 .
GeneIDi 1499318.
KEGGi bfl:Bfl118.
PATRICi 31963831. VBICanBlo38691_0118.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BFLO203907:GHF7-118-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYI_BLOFL
AccessioniPrimary (citable) accession number: Q7VQL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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