Reviewed, UniProtKB/Swiss-Prot Q7VQJ7 (LEU3_BLOFL)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	Bfl132

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	3-isopropylmalate dehydrogenase		PRO_0000083647
Regions
	Nucleotide binding	79 – 92	14	NAD By similarity
	Nucleotide binding	286 – 298	13	NAD By similarity
Sites
	Metal binding	228	1	Magnesium or manganese By similarity
	Metal binding	252	1	Magnesium or manganese By similarity
	Metal binding	256	1	Magnesium or manganese By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	228	1	Substrate By similarity
	Site	146	1	Important for catalysis By similarity
	Site	196	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VQJ7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 144A37D7F5B028F6	FASTA	364	40,485
	10 20 30 40 50 60 MNNNTYHIAI LPGDGIGPEI MQQAYKILNT VKNKFKINII TTEYKVGGNA LDLEGSPLPK 70 80 90 100 110 120 DTIKNCEKSN AILFGAVGGP KWNNINETSR PEFGALLTLR KHFNLFINLR PIYLPIELIN 130 140 150 160 170 180 LSPLKPEIIS QGLDMICIRE LIGGIYFGKP QGKSGIRSQE HAFDTAIYHR FEIERIAHFA 190 200 210 220 230 240 FKLAQKRRKH VTSIDKANVL HTSMLWRKVV SEVASNYPNV HLQHLYVDNA SMQLIRNPSA 250 260 270 280 290 300 FDVILCPNLF GDILSDECAE INGSIGILPS ASLNEHNFGL YEPAGGSAPD IAGKNIANPI 310 320 330 340 350 360 AQILSTALLF RYSLKLNHVA ITIEKAVYKA LTLGYRTQDI AYNKQKSVNT DDMGDIIASL IYKL

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83653.1.
RefSeq	NP_878438.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1499332.
GenomeReviews	Gene locus Bfl132 in contig BX248583_GR.
KEGG	bfl:Bfl132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VQJ7.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL132-MON.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_BLOFL

Accession

Primary (citable) accession number: Q7VQJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	November 4, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)