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Q7VQJ2 (MURE_BLOFL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
EC=6.3.2.13
Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase
Gene names
Name:murE
Ordered Locus Names:Bfl137
OrganismBlochmannia floridanus [Complete proteome] [HAMAP]
Taxonomic identifier203907 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP-Rule MF_00208

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP-Rule MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00208

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00208.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP-Rule MF_00208

Sequence similarities

Belongs to the MurCDEF family. MurE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP-Rule MF_00208
PRO_0000101877

Regions

Nucleotide binding122 – 1287ATP Potential
Region164 – 1652UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region421 – 4244Meso-diaminopimelate binding By similarity
Motif421 – 4244Meso-diaminopimelate recognition motif HAMAP-Rule MF_00208

Sites

Binding site301UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity
Binding site321UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1911UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1971UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1991UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site3971Meso-diaminopimelate By similarity
Binding site4721Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site4761Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2311N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VQJ2 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: AB6777C321FC7458

FASTA49956,189
        10         20         30         40         50         60 
MFKLKQLHEL IVPWISGINV KIPYLTGLEL DSRNINFGNL FIAIQGYNTD GRLHIDDAIN 

        70         80         90        100        110        120 
NGAVAILSES YNKSTFFIKK IVNNVEVIPI IYFNQLNKCI SNIAGRFYDH PSLCLKLIGV 

       130        140        150        160        170        180 
TGTNGKTTIT HLLTNWTSLL GERSAVMGTL GNGVLSDINP SCCTTCSAIE TQKILKQFVQ 

       190        200        210        220        230        240 
SGVTFVAMEI SSHGLDQYRV DSLYFDVAIF SNISNDHLDY HNNINQYRMA KWRLFNELRV 

       250        260        270        280        290        300 
KNYVINVDDC VGYRWLLSLS NAVAVTIKNN LPRSWSGRWI ALVKADYYLY GTKIVFKSSW 

       310        320        330        340        350        360 
GNGFIHSQLL GEVNVSNILL ALGALLIMGY SLKSLLYTGS KLYPVCGRLE VLSCLNHPTV 

       370        380        390        400        410        420 
IVDYAHTPDA LKKILIFAKH LCNKGRLWCI FGCGGNRDRS KRSLMGVIAN RYSDYVIITN 

       430        440        450        460        470        480 
DNPRVEDPKL IIDDIICKIR NSEKLKIIED RVYAINMVVS KACSDDFVLI LGKGHEKYQN 

       490 
IGLNYISHSD QDIVKSIFK

« Hide

References

[1]"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes."
Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX248583 Genomic DNA. Translation: CAD83658.1.
RefSeqNP_878443.1. NC_005061.1.

3D structure databases

ProteinModelPortalQ7VQJ2.
ModBaseSearch...

Protein-protein interaction databases

STRING203907.Bfl137.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD83658; CAD83658; Bfl137.
GeneID1499337.
KEGGbfl:Bfl137.
PATRIC31963867. VBICanBlo38691_0136.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0769.
HOGENOMHOG000268118.
KOK01928.
OMAGALAYVD.
ProtClustDBPRK00139.

Enzyme and pathway databases

BioCycBFLO203907:GHF7-137-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPMF_00208. MurE.
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01085. murE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURE_BLOFL
AccessionPrimary (citable) accession number: Q7VQJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: October 1, 2003
Last modified: May 29, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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