Reviewed, UniProtKB/Swiss-Prot Q7VQJ2 (MURE_BLOFL)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	Bfl137

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_0000101877
Regions
	Nucleotide binding	122 – 128	7	ATP Potential
	Region	164 – 165	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	421 – 424	4	Meso-diaminopimelate binding By similarity
	Motif	421 – 424	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	30	1	UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity
	Binding site	32	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	191	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	197	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	199	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	397	1	Meso-diaminopimelate By similarity
	Binding site	472	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	476	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	231	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VQJ2-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: AB6777C321FC7458	FASTA	499	56,189
	10 20 30 40 50 60 MFKLKQLHEL IVPWISGINV KIPYLTGLEL DSRNINFGNL FIAIQGYNTD GRLHIDDAIN 70 80 90 100 110 120 NGAVAILSES YNKSTFFIKK IVNNVEVIPI IYFNQLNKCI SNIAGRFYDH PSLCLKLIGV 130 140 150 160 170 180 TGTNGKTTIT HLLTNWTSLL GERSAVMGTL GNGVLSDINP SCCTTCSAIE TQKILKQFVQ 190 200 210 220 230 240 SGVTFVAMEI SSHGLDQYRV DSLYFDVAIF SNISNDHLDY HNNINQYRMA KWRLFNELRV 250 260 270 280 290 300 KNYVINVDDC VGYRWLLSLS NAVAVTIKNN LPRSWSGRWI ALVKADYYLY GTKIVFKSSW 310 320 330 340 350 360 GNGFIHSQLL GEVNVSNILL ALGALLIMGY SLKSLLYTGS KLYPVCGRLE VLSCLNHPTV 370 380 390 400 410 420 IVDYAHTPDA LKKILIFAKH LCNKGRLWCI FGCGGNRDRS KRSLMGVIAN RYSDYVIITN 430 440 450 460 470 480 DNPRVEDPKL IIDDIICKIR NSEKLKIIED RVYAINMVVS KACSDDFVLI LGKGHEKYQN 490 IGLNYISHSD QDIVKSIFK

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83658.1.
RefSeq	NP_878443.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VQJ2.
Genome annotation databases
GeneID	1499337.
GenomeReviews	Gene locus Bfl137 in contig BX248583_GR.
KEGG	bfl:Bfl137.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VQJ2.
OMA	HTPDGIE.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL137-MON.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_BLOFL

Accession

Primary (citable) accession number: Q7VQJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)