Reviewed, UniProtKB/Swiss-Prot Q7VQI7 (MURC_BLOFL)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Bfl143

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182075
Regions
	Nucleotide binding	127 – 133	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7VQI7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 2C8C6455543D369F	FASTA	480	54,194
	10 20 30 40 50 60 MQSSYFKNGI LPIINRFYTK QIHFVGIGGI GMCGLAMLLL AQGYCITGSD IAQNDMIRSL 70 80 90 100 110 120 LKLGIKIFFG HRDSNINQEI NIVVISSAID INNPEVQQAK RLKIPVIHRV KILSELMRYK 130 140 150 160 170 180 YGIAIAGTHG KTTTVSMLTN IYVEAGLDPT FVNGGIIRSQ GVSARLGYGN DFIAESDESD 190 200 210 220 230 240 KSFLRLCPVV AVITNIDTDH MNTYHQNFEY LQEVFIKFLD KLPMYGYAVV CIDDPVVRKI 250 260 270 280 290 300 LPRINRKIIT YGFSKDATLC IVKYYQYREK SNFTILIKNN KELQVVLNTP GYHNALNAAA 310 320 330 340 350 360 SIAVAIEEGI RDTVILKSMS NFTGTNRRFE FLGNYPLNII NGRVGKVMVI DDYGHHPAEL 370 380 390 400 410 420 RSTIIAIRTG WSDHRLVMVF QPHRFTRTHE LYDNFVRVLS TVDVLLMLDI YSAGEQPIMG 430 440 450 460 470 480 INAQSLCDSI LKYAKVQPIF VQNECFLLKF LMNLLRDKDL LLIQGAGTIS EIVKALFIKR

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248583 Genomic DNA. Translation: CAD83664.1.
RefSeq	NP_878449.1.
3D structure databases
SMR	Q7VQI7. Positions 10-476.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VQI7.
Genome annotation databases
GeneID	1499343.
GenomeReviews	Gene locus Bfl143 in contig BX248583_GR.
KEGG	bfl:Bfl143.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HBG476594.
OMA	YQPHRYS.
PhylomeDB	Q7VQI7.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL143-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BLOFL

Accession

Primary (citable) accession number: Q7VQI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)