Reviewed, UniProtKB/Swiss-Prot Q7VQH2 (CYSJ_BLOFL)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] flavoprotein alpha-component
      Short name=SIR-FP
    EC=1.8.1.2

Gene names

Name:	cysJ
Ordered Locus Names:	Bfl158

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavo-protein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 FAD per subunit By similarity. Binds 1 FMN per subunit By similarity.
Subunit structure	Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis Electron transport Transport
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 610	610	Sulfite reductase [NADPH] flavoprotein alpha-component		PRO_0000199922
Regions
	Domain	68 – 206	139	Flavodoxin-like
	Domain	243 – 459	217	FAD-binding FR-type
	Nucleotide binding	74 – 78	5	FMN By similarity
	Nucleotide binding	154 – 185	32	FMN By similarity
	Nucleotide binding	245 – 297	53	FAD By similarity
	Nucleotide binding	483 – 610	128	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VQH2-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 1D5507E560F6C562	FASTA	610	69,495
	10 20 30 40 50 60 MSIMGLLSSE QLNQLKLVLD TLSKNQLIWL SGYLLGLVNS QTSTDIGVTA SGVSAGLELK 70 80 90 100 110 120 PDLIDNTIIV ISASQTGNAR NIAKQLYSDL VEAGLRAVLF SAGEYKFKKI SEISLLIFIT 130 140 150 160 170 180 STHGEGEPPE EALALYKYLF SEKALRMEKT SFIVLSLGDR SYEYFAKAGK DFDKRFEDLG 190 200 210 220 230 240 ANRLYDRVDL DVDFQSEVDK WKEKVVSLCK SKIVSIVSKD KCINIQNNIV FKKKNPVTSY 250 260 270 280 290 300 CKEFPLIAYL LNRQKITSCN SLKDVHHLEF DISGSGLCYQ PGDALGIWYE NDYNLVYELL 310 320 330 340 350 360 ELLNLTGRES VQIKNQSMCL DEALVKYCDL TQNTPVVVKS IATISQDKIL LNLLQNQNQL 370 380 390 400 410 420 NSFCSTTPIV EMFYQISMTK QLSSQELIQI LRPMRPRFYS IASAQSEVGE EIHITVSVVR 430 440 450 460 470 480 YTINGRIRSG GASSYLVDRV QDHDEIRIFV ESNDNFRLPK DPNVSIIMIG AGTGIAPFRS 490 500 510 520 530 540 FMQQRALDKA LGKNWLFFGN LKFTDDFLYQ IEWKTYFKSG ILNKIDTAWS RDQDYKVYVQ 550 560 570 580 590 600 DKLLSNGLEL WDWIQKGAYI YVCGDAKYMA RDVEQALVTV VSIHGNMNMD QSNDFWNEMR 610 VQHRYQRDIY

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83679.1.
RefSeq	NP_878464.1.
3D structure databases
HSSP	HSSP built from PDB template 1DDG based on UniProtKB P38038.
ModBase	Search...
Genome annotation databases
GeneID	1499358.
GenomeReviews	Gene locus Bfl158 in contig BX248583_GR.
KEGG	bfl:Bfl158.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VQH2.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL158-MON.
Family and domain databases
HAMAP	MF_01541. [Tree]
InterPro	IPR010199. CysJ. IPR003097. FAD-binding_1. IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR001709. FPN_cyt_redctse. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
TIGRFAMs	TIGR01931. cysJ. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSJ_BLOFL

Accession

Primary (citable) accession number: Q7VQH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)