Q7VQF2 (MURB_BLOFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	Bfl183

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179192
Regions
	Domain	15 – 218	204	FAD-binding PCMH-type
Sites
	Active site	161	1	By similarity
	Active site	230	1	Proton donor By similarity
	Active site	327	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VQF2 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 3B63619602708EAD	FASTA	345	39,756
	10 20 30 40 50 60 MKRCTKLKIL NTFSVDVYAE KVIIINDEYS LLQLWKQSRD QDKFFLILGA GSNVLFLENY 70 80 90 100 110 120 KGIVLLNRIK GIFVTENKVA WYLHVGAGEE WNTLVMYTIK RNMPGLENLV CIPGYVGAAL 130 140 150 160 170 180 IQNIGAYGVE LSQMCEYVDV LDLNQGDKIR LYCHECCFRY RESIFKLNLY KYAILFVGLR 190 200 210 220 230 240 INKHWKPVLS YSGLTHLNLN SITPRQIINT IIFLRYKKLP NPIIHGNVGS FFKNPVVDFK 250 260 270 280 290 300 VVSFLLKKYS NIPYYFQEDG KVKLLAGWLI ENCNLKGYIL GEASVYYKQA LVLINTRQKA 310 320 330 340 TGTEIAALAL YVYNKVVDKF NIRLKPEVRL IGSFGEINPK KLFLK

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248583 Genomic DNA. Translation: CAD83702.1.
RefSeq	NP_878486.1. NC_005061.1.
3D structure databases
ProteinModelPortal	Q7VQF2.
SMR	Q7VQF2. Positions 7-341.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VQF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1499383.
GenomeReviews	Gene locus Bfl183 in contig BX248583_GR.
KEGG	bfl:Bfl183.
PATRIC	31963961. VBICanBlo38691_0181.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HBG686573.
OMA	LAYEITS.
PhylomeDB	Q7VQF2.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL183-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BLOFL

Accession

Primary (citable) accession number: Q7VQF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program