Reviewed, UniProtKB/Swiss-Prot Q7VQB7 (GLO2_BLOFL)

Names and origin

Protein names

Recommended name:
    Hydroxyacylglutathione hydrolase
    EC=3.1.2.6
Alternative name(s):
    Glyoxalase II
      Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Bfl223

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309627
Sites
	Metal binding	53	1	Zinc 1 By similarity
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	57	1	Zinc 2 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	111	1	Zinc 1 By similarity
	Metal binding	128	1	Zinc 1 By similarity
	Metal binding	128	1	Zinc 2 By similarity
	Metal binding	166	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VQB7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 9D9BF1C8DA584E98	FASTA	256	29,582
	10 20 30 40 50 60 MNIIKCISLQ TNYIWIIYNH IRQCIIIDPG ESLKVIKMLN HLQLIPEAIL LTHNHRDHTN 70 80 90 100 110 120 GVFTLIQHFP KIKIYGPIET QNQNFATITV SEGDTLQILN QEIKVLNFPG HTPHHIGFYY 130 140 150 160 170 180 KSWLFSGDTI FSAGCGTIQT GYTQAMYKSL LKIQCLPKNT LIYSGHEYTL LNLNFAISIL 190 200 210 220 230 240 PQDTLIINYL KKITKVLKKN KLLQPTTLQL ELKINPFLRC NDINIQKSLN LFPKPGEEWK 250 ILQALRNKKD LYLVKN

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248583 Genomic DNA. Translation: CAD83737.1.
RefSeq	NP_878521.1.
3D structure databases
HSSP	HSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
SMR	Q7VQB7. Positions 1-252.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7VQB7.
Genome annotation databases
GeneID	1499423.
GenomeReviews	Gene locus Bfl223 in contig BX248583_GR.
KEGG	bfl:Bfl223.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	ICAGHEY.
PhylomeDB	Q7VQB7.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL223-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Blactmase-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_BLOFL

Accession

Primary (citable) accession number: Q7VQB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)