Reviewed, UniProtKB/Swiss-Prot Q7VQB1 (TGT_BLOFL)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	Bfl230

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_0000135461
Sites
	Active site	89	1	Nucleophile By similarity
	Metal binding	302	1	Zinc By similarity
	Metal binding	304	1	Zinc By similarity
	Metal binding	307	1	Zinc By similarity
	Metal binding	334	1	Zinc By similarity
	Binding site	90	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VQB1-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 99C8ECE4ADAAACDD	FASTA	378	43,608
	10 20 30 40 50 60 MVFRVLQTDG DARVGKLTCH HGIIETPAFI PVGTYGVVKS VTSQEVAESG AQIILSNTFH 70 80 90 100 110 120 LWLRPGLEII KIHQNLHKFM NWMGPIITDS GGFQVFSLNK LRKITESGVY FKDPVNGSTI 130 140 150 160 170 180 FLTPEKSMDI QYHLGSDIVL VFDECVSYPS TWEYIKNSVE ISLNWAERSR LHFDKLHNSN 190 200 210 220 230 240 MLFAIIQGGM YEELRNRSAQ ELINIKFDGY AIGGLSVGET PQERFRIVSY VCKIIPFDKP 250 260 270 280 290 300 RYLMGVGKPQ DLIEAVCLGV DMFDCVIPTR NARNGYLFTN NGVVRIRNAK YESDLDPIEE 310 320 330 340 350 360 DCDCYTCQRY YSRSYLHYLD RCNESLGIRL NTIHNLRYYQ RLMEEIRQSI RMKKLRKFVE 370 AFNYKFNSVS TNNYLSNI

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83743.1.
RefSeq	NP_878527.1.
3D structure databases
HSSP	HSSP built from PDB template 1PXG based on UniProtKB P28720.
ModBase	Search...
Genome annotation databases
GeneID	1499430.
GenomeReviews	Gene locus Bfl230 in contig BX248583_GR.
KEGG	bfl:Bfl230.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VQB1.
OMA	Q7VQB1. VLNSDIV.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL230-MON.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_BLOFL

Accession

Primary (citable) accession number: Q7VQB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)