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Q7VPJ7

- NAPA_HAEDU

UniProt

Q7VPJ7 - NAPA_HAEDU

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciHDUC233412:GH5F-75-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:HD_0074
    OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
    Taxonomic identifieri233412 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000001022: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
    BLAST
    Chaini33 – 827795Periplasmic nitrate reductasePRO_0000045987Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi233412.HD0074.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7VPJ7.
    SMRiQ7VPJ7. Positions 36-826.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    KOiK02567.
    OMAiAGKMHDS.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q7VPJ7-1 [UniParc]FASTAAdd to Basket

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    MELSRRDFMK ANAAVAAAAA AGIVLPVKNV QADDSGIKWD KAPCRFCGTG    50
    CSVLVGTKDG RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQTPLL 100
    RMKDGKFHKE GDFTPVSWDQ AFSIMAEKVK ATLKAKGGNA VGMFSSGQTT 150
    IFEGYAKVKL WKAGLRSNTI DPNARHCMAS AAVAFLRTFG MDEPMGCYND 200
    IEKTDGFVLW GSNMAEMHPI LWSRISDRRL SDNNVKVVVM STFEHRSFEL 250
    ADTPIIFKPH SDLAILNYIA NYIIQNDKVN WDFVNKHTKF KRGETDIGYG 300
    LRPEHPLQKA AKNVKNAGKM HDSSFEEFKK IVEPYTLEKA HEISGVPKHQ 350
    LEALAKMYAD PAQKLVSFWT MGFNQHTRGV WVNHMIYNVH LLTGKISTPG 400
    CGPFSLTGQP SACGTAREVG TFVHRLPADM VVMNPKHVEI CEKAWKLPKG 450
    TIPTVPGYAA VMQSRMLKDG KLNFLWQMCT NNMQGGPNIN EEIFPGWRNP 500
    ENFIVVSDPY PSVSAVAADL ILPTCMWVEK EGAYGNAERR TQFWYQQVKG 550
    PAQSKSDLWQ IVEFAKYFKA EEVWNEELMA QMPEYRGKTL YEILYLNGEV 600
    DKYQVPTNIP GYLNDEADDF GFYIQKGLFE EYAQFGRGHG HDLADFETYH 650
    QARGLRWPVV DGKETLWRYR EGFDPYVKAG EDFRFYGYPD GKAIILGVPY 700
    ESPAESPDEE YDLWLSTGRV LEHWHTGTMT RRVPELHRSF PNNLVWMHPS 750
    DAKKRGLRHG DKVKVITRRG EMISHLDTRG RNKCPEGLIF TTFFDAGQLA 800
    NKLTLDATDP ISGETDFKKC AAKVVKA 827
    Length:827
    Mass (Da):93,202
    Last modified:October 1, 2003 - v1
    Checksum:i85716F1C2E2D9D5E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017143 Genomic DNA. Translation: AAP95084.1.
    RefSeqiNP_872695.1. NC_002940.2.
    WP_010944138.1. NC_002940.2.

    Genome annotation databases

    EnsemblBacteriaiAAP95084; AAP95084; HD_0074.
    GeneIDi1490097.
    KEGGihdu:HD0074.
    PATRICi20176777. VBIHaeDuc133973_0057.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017143 Genomic DNA. Translation: AAP95084.1 .
    RefSeqi NP_872695.1. NC_002940.2.
    WP_010944138.1. NC_002940.2.

    3D structure databases

    ProteinModelPortali Q7VPJ7.
    SMRi Q7VPJ7. Positions 36-826.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233412.HD0074.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP95084 ; AAP95084 ; HD_0074 .
    GeneIDi 1490097.
    KEGGi hdu:HD0074.
    PATRICi 20176777. VBIHaeDuc133973_0057.

    Phylogenomic databases

    eggNOGi COG0243.
    KOi K02567.
    OMAi AGKMHDS.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci HDUC233412:GH5F-75-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Haemophilus ducreyi."
      Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 35000HP / ATCC 700724.

    Entry informationi

    Entry nameiNAPA_HAEDU
    AccessioniPrimary (citable) accession number: Q7VPJ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3