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Q7VPJ7 (NAPA_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:HD_0074
OrganismHaemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]
Taxonomic identifier233412 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 827795Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045987

Regions

Domain37 – 93574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding441Iron-sulfur (4Fe-4S) By similarity
Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding791Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VPJ7 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 85716F1C2E2D9D5E

FASTA82793,202
        10         20         30         40         50         60 
MELSRRDFMK ANAAVAAAAA AGIVLPVKNV QADDSGIKWD KAPCRFCGTG CSVLVGTKDG 

        70         80         90        100        110        120 
RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQTPLL RMKDGKFHKE GDFTPVSWDQ 

       130        140        150        160        170        180 
AFSIMAEKVK ATLKAKGGNA VGMFSSGQTT IFEGYAKVKL WKAGLRSNTI DPNARHCMAS 

       190        200        210        220        230        240 
AAVAFLRTFG MDEPMGCYND IEKTDGFVLW GSNMAEMHPI LWSRISDRRL SDNNVKVVVM 

       250        260        270        280        290        300 
STFEHRSFEL ADTPIIFKPH SDLAILNYIA NYIIQNDKVN WDFVNKHTKF KRGETDIGYG 

       310        320        330        340        350        360 
LRPEHPLQKA AKNVKNAGKM HDSSFEEFKK IVEPYTLEKA HEISGVPKHQ LEALAKMYAD 

       370        380        390        400        410        420 
PAQKLVSFWT MGFNQHTRGV WVNHMIYNVH LLTGKISTPG CGPFSLTGQP SACGTAREVG 

       430        440        450        460        470        480 
TFVHRLPADM VVMNPKHVEI CEKAWKLPKG TIPTVPGYAA VMQSRMLKDG KLNFLWQMCT 

       490        500        510        520        530        540 
NNMQGGPNIN EEIFPGWRNP ENFIVVSDPY PSVSAVAADL ILPTCMWVEK EGAYGNAERR 

       550        560        570        580        590        600 
TQFWYQQVKG PAQSKSDLWQ IVEFAKYFKA EEVWNEELMA QMPEYRGKTL YEILYLNGEV 

       610        620        630        640        650        660 
DKYQVPTNIP GYLNDEADDF GFYIQKGLFE EYAQFGRGHG HDLADFETYH QARGLRWPVV 

       670        680        690        700        710        720 
DGKETLWRYR EGFDPYVKAG EDFRFYGYPD GKAIILGVPY ESPAESPDEE YDLWLSTGRV 

       730        740        750        760        770        780 
LEHWHTGTMT RRVPELHRSF PNNLVWMHPS DAKKRGLRHG DKVKVITRRG EMISHLDTRG 

       790        800        810        820 
RNKCPEGLIF TTFFDAGQLA NKLTLDATDP ISGETDFKKC AAKVVKA 

« Hide

References

[1]"The complete genome sequence of Haemophilus ducreyi."
Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017143 Genomic DNA. Translation: AAP95084.1.
RefSeqNP_872695.1. NC_002940.2.

3D structure databases

ProteinModelPortalQ7VPJ7.
SMRQ7VPJ7. Positions 36-826.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233412.HD0074.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP95084; AAP95084; HD_0074.
GeneID1490097.
KEGGhdu:HD0074.
PATRIC20176777. VBIHaeDuc133973_0057.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
KOK02567.
OMAAGKMHDS.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycHDUC233412:GH5F-75-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_HAEDU
AccessionPrimary (citable) accession number: Q7VPJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families