Q7VPJ7 (NAPA_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase
EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	HD_0074

Organism

Haemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]

Taxonomic identifier

233412 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Tat-type signal Potential
	Chain	33 – 827	795	Periplasmic nitrate reductase HAMAP MF_01630		PRO_0000045987
Sites
	Metal binding	44	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	47	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	79	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VPJ7 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 85716F1C2E2D9D5E	FASTA	827	93,202
	10 20 30 40 50 60 MELSRRDFMK ANAAVAAAAA AGIVLPVKNV QADDSGIKWD KAPCRFCGTG CSVLVGTKDG 70 80 90 100 110 120 RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQTPLL RMKDGKFHKE GDFTPVSWDQ 130 140 150 160 170 180 AFSIMAEKVK ATLKAKGGNA VGMFSSGQTT IFEGYAKVKL WKAGLRSNTI DPNARHCMAS 190 200 210 220 230 240 AAVAFLRTFG MDEPMGCYND IEKTDGFVLW GSNMAEMHPI LWSRISDRRL SDNNVKVVVM 250 260 270 280 290 300 STFEHRSFEL ADTPIIFKPH SDLAILNYIA NYIIQNDKVN WDFVNKHTKF KRGETDIGYG 310 320 330 340 350 360 LRPEHPLQKA AKNVKNAGKM HDSSFEEFKK IVEPYTLEKA HEISGVPKHQ LEALAKMYAD 370 380 390 400 410 420 PAQKLVSFWT MGFNQHTRGV WVNHMIYNVH LLTGKISTPG CGPFSLTGQP SACGTAREVG 430 440 450 460 470 480 TFVHRLPADM VVMNPKHVEI CEKAWKLPKG TIPTVPGYAA VMQSRMLKDG KLNFLWQMCT 490 500 510 520 530 540 NNMQGGPNIN EEIFPGWRNP ENFIVVSDPY PSVSAVAADL ILPTCMWVEK EGAYGNAERR 550 560 570 580 590 600 TQFWYQQVKG PAQSKSDLWQ IVEFAKYFKA EEVWNEELMA QMPEYRGKTL YEILYLNGEV 610 620 630 640 650 660 DKYQVPTNIP GYLNDEADDF GFYIQKGLFE EYAQFGRGHG HDLADFETYH QARGLRWPVV 670 680 690 700 710 720 DGKETLWRYR EGFDPYVKAG EDFRFYGYPD GKAIILGVPY ESPAESPDEE YDLWLSTGRV 730 740 750 760 770 780 LEHWHTGTMT RRVPELHRSF PNNLVWMHPS DAKKRGLRHG DKVKVITRRG EMISHLDTRG 790 800 810 820 RNKCPEGLIF TTFFDAGQLA NKLTLDATDP ISGETDFKKC AAKVVKA

References

[1]

"The complete genome sequence of Haemophilus ducreyi." Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017143 Genomic DNA. Translation: AAP95084.1.
RefSeq	NP_872695.1. NC_002940.2.
3D structure databases
ProteinModelPortal	Q7VPJ7.
SMR	Q7VPJ7. Positions 36-826.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1490097.
GenomeReviews	Gene locus HD_0074 in contig AE017143_GR.
KEGG	hdu:HD0074.
NMPDR	fig|233412.1.peg.60.
PATRIC	20176777. VBIHaeDuc133973_0057.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG715033.
OMA	NMLYNIH.
ProtClustDB	PRK13532.
Enzyme and pathway databases
BioCyc	HDUC233412:HD_0074-MONOMER.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
KO	K02567.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_HAEDU

Accession

Primary (citable) accession number: Q7VPJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program