Q7VP66 (CARA_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase small chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	HD_0235

Organism

Haemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]

Taxonomic identifier

233412 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112279
Regions
	Domain	203 – 389	187	Glutamine amidotransferase type-1
	Region	1 – 199	199	CPSase HAMAP MF_01209
Sites
	Active site	279	1	Nucleophile By similarity
	Active site	363	1	By similarity
	Active site	365	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VP66 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7553218FAB9F38C6	FASTA	389	42,748
	10 20 30 40 50 60 MFNPAILVLA DGSTFYGKSI GYQAVSAYSI GEVVFNTAMT GYQEILTDPS YAKQIVTLTY 70 80 90 100 110 120 PHIGNTGTND EDNESNQIYA SGLIIRDLPL LHSNFRATSS LSDYLIQHNI IAIADIDTRR 130 140 150 160 170 180 LTRILRETGS QAGCIYIPNE NESLAQAVEK AQDLAKSFGS MAGKDLAKEV TCQHRYEWQE 190 200 210 220 230 240 GIWHLPTNNA GKPFNLQTTP TFHVVAYDFG IKQNILRMLA ERGCKITVVP AQTSAEDILA 250 260 270 280 290 300 LNPDGIFLSN GPGDPEPCTY AIQSIRQLLN TQKPIFGICL GHQLLGLAVG AKTRKMAFGH 310 320 330 340 350 360 HGANHPVQDL KTEHVLITSQ NHGFEIDEYS LPENVIVTHR SLFDNSVQGI ELTNQVAFSF 370 380 QGHPEANPGP QDVAYLFDKF INAVQATKA

References

[1]

"The complete genome sequence of Haemophilus ducreyi." Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017143 Genomic DNA. Translation: AAP95221.1.
RefSeq	NP_872832.1. NC_002940.2.
3D structure databases
ProteinModelPortal	Q7VP66.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1490239.
GenomeReviews	Gene locus HD_0235 in contig AE017143_GR.
KEGG	hdu:HD0235.
NMPDR	fig|233412.1.peg.197.
PATRIC	20177041. VBIHaeDuc133973_0188.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286341.
OMA	FTYPELG.
ProtClustDB	PRK12564.
Enzyme and pathway databases
BioCyc	HDUC233412:HD_0235-MONOMER.
Family and domain databases
HAMAP	MF_01209. CPSase_S_chain. [Tree]
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_HAEDU

Accession

Primary (citable) accession number: Q7VP66

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program