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Reviewed, UniProtKB/Swiss-Prot Q7VP59 (MURE_HAEDU)

Last modified November 3, 2009. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase
Gene names
Name: murE
Ordered Locus Names: HD_0242
OrganismHaemophilus ducreyi [Complete proteome] [HAMAP]
Taxonomic identifier730 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

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Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.

Sequence similarities

Belongs to the murCDEF family. MurE subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208
PRO_0000101899

Regions

Nucleotide binding123 – 1297ATP Potential
Region43 – 453UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region165 – 1662UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region422 – 4254Meso-diaminopimelate binding By similarity
Motif422 – 4254Meso-diaminopimelate recognition motif HAMAP MF_00208

Sites

Binding site261UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity
Binding site281UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1641UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1921UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1981UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site2001UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site3981Meso-diaminopimelate By similarity
Binding site4731Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site4771Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2321N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q7VP59-1 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: A289AB0DCFAFE15D

FASTA50155,125
        10         20         30         40         50         60 
MKRLLPFLTD LEAWVEQLIP LKQMTLDSRQ VTEGDLFIAL KGHQCDGRQF IQNAIEQGAA 

        70         80         90        100        110        120 
IILAEAESDQ DEIELDSQFA RYNLDRRACK VITVPRLAER LSAIADSFYA SPSAKLKLIG 

       130        140        150        160        170        180 
ITGTNGKTTT AQLLAQWHNL LGGHSAVMGT IGNGLYGQEQ EAINTTGSAI EVQQNLARFV 

       190        200        210        220        230        240 
EQGADFCAME VSSHGLAQYR VEALQYDLAI FTNLSRDHLD YHHSIAAYEA AKFRLFNALQ 

       250        260        270        280        290        300 
TKAQVLNADD QVAQNWLSML PNAVLVSCDP NFTSEHQFVK ATKVNFSLQG AYIEFESSWG 

       310        320        330        340        350        360 
NGQFHSQLSG AFNVTNILLA LAGLLTLGYD LAKLVATASQ LRSVTGRMQK VSAITDKNRP 

       370        380        390        400        410        420 
LVLVDYAHTP DALQKALQAA RLHTKGKLFC VFGCGGDRDC GKRPLMATIA EELADGVIVT 

       430        440        450        460        470        480 
NDNPRTEDQH KIVAEIMQGF AKPDNILVIY DREQAIQHAI KHASSADLIL IAGKGHENYQ 

       490        500 
IIGTIKHHFS DQEIASKYLS Q

« Hide

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References

[1]"The complete genome sequence of Haemophilus ducreyi."
Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 35000HP / ATCC 700724.

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Cross-references

Sequence databases

AE017143 Genomic DNA. Translation: AAP95228.1.
RefSeqNP_872839.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1490246.
GenomeReviewsGene locus HD_0242 in contig AE017143_GR.
KEGGhdu:HD0242.
NMPDRfig|233412.1.peg.204.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ7VP59.
OMAFPVIVDY.

Enzyme and pathway databases

BioCycHDUC233412:HD_0242-MON.
BRENDA6.3.2.13. 265031.

Family and domain databases

HAMAPMF_00208.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
TIGRFAMsTIGR01085. murE. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameMURE_HAEDU
AccessionPrimary (citable) accession number: Q7VP59
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: October 1, 2003
Last modified: November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents