Q7VP41 (MDH_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	HD_0264

Organism

Haemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]

Taxonomic identifier

233412 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus ducreyi

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Malate dehydrogenase HAMAP-Rule MF_01516		PRO_0000113305
Regions
	Nucleotide binding	7 – 13	7	NAD By similarity
	Nucleotide binding	124 – 126	3	NAD By similarity
Sites
	Active site	184	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	101	1	NAD By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	238	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VP41 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: CAE3E8E3D351AAFB	FASTA	324	34,248
	10 20 30 40 50 60 MKVTVLGAAG GIGQTLSLLL KLRLPAGADL ALYDISPVTP GIAVDISHIP TSVNAIGYAG 70 80 90 100 110 120 EDPSVALKDP SVALKDTNLV IITAGIARKP GMTRADLFNI NAGIVKDLVE KVAEVCPMAC 130 140 150 160 170 180 IGIVTNPVNT LVPIAAEVLR KAGVYDKSKL FGVTTLDVVR AKTFVSELKH KNVETVTVPV 190 200 210 220 230 240 VGGHSGTTIL PLLSQALAEG RLLDFSQTEI ESLTQRIKNA GTEVVEAKAG GGSATLSMAE 250 260 270 280 290 300 SGARFALAVF KALLGEDCVR YAYVQSKEGS GYPEFFAHPV RFGLKGIEEI LPIAPLNEYE 310 320 QTQFEELKPI LEADIELGKK FVNP

References

[1]

"The complete genome sequence of Haemophilus ducreyi." Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017143 Genomic DNA. Translation: AAP95246.1.
RefSeq	NP_872857.1. NC_002940.2.
3D structure databases
ProteinModelPortal	Q7VP41.
SMR	Q7VP41. Positions 1-323.
ModBase	Search...
Protein-protein interaction databases
STRING	233412.HD0264.
Proteomic databases
PRIDE	Q7VP41.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP95246; AAP95246; HD_0264.
GeneID	1490265.
KEGG	hdu:HD0264.
PATRIC	20177093. VBIHaeDuc133973_0214.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
KO	K00024.
OMA	VEVKGFA.
ProtClustDB	PRK05086.
Enzyme and pathway databases
BioCyc	HDUC233412:GH5F-231-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01516. Malate_dehydrog_1.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR023958. Malate_DH_type1_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit. PTHR11540:SF1. PTHR11540:SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_HAEDU

Accession

Primary (citable) accession number: Q7VP41

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program