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Q7VP34

- SYI_HAEDU

UniProt

Q7VP34 - SYI_HAEDU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
    Binding sitei603 – 6031ATPUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHDUC233412:GH5F-250-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:HD_0272
    OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
    Taxonomic identifieri233412 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000001022: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 935935Isoleucine--tRNA ligasePRO_0000098394Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi233412.HD0272.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7VP34.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7VP34-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDYKDTLNL PETGFPMRGD LAKREPAMLK NWYDKNLYQQ IRQTSKGKKT    50
    FILHDGPPYA NGNLHLGHAV NKILKDIIMK SKTASGFDTP YVPGWDCHGL 100
    PIELKVESIV GKPNEKISAA EFRQACREYA NEQVDRQKAD FIRMGVLGDW 150
    DNPYLTMNFN TEASIIRTLA KVIANGHLYK GSKPVHWCLD CGSSLAEAEV 200
    EYEDKVSPSI YVRFKACDET AIETLFNAKG NGPISAIIWT TTPWTMPSNR 250
    AIAIHPELEY ALVQLADERV ILATELVESV AKAIEAESFD ILATVKGETL 300
    QLLRFHHPFY AFDVPFIFGD HVTTEGGTGL VHTAPDHGTD DFIIARKNNI 350
    EMAGLIGNDG KFKSDVEFFA GLAVFESNEK VIEKLKETGA LLKLARIKHS 400
    YPHCWRHKTP IIFRATPQWF IGMEKQGLRA QALAEIKTVR WIPNWGEARI 450
    DTMVANRPDW CISRQRTWGV PMAMFVHNET EELHPRTLDI LELVAQRVEQ 500
    QGIQAWWDLD PTEVLGEDAQ HYHKVPDTLD VWFDSGSTYA SVVEQRPEFN 550
    GQTADMYLEG SDQHRGWFMS SLMLASATNG KAPYQQVLTH GFVVDEKGRK 600
    MSKSIGNVIV PSEVWNKNGA DILRLWVAST DYTAEMKVSH GILNSAGDAY 650
    RRIRNTARFL LSNLNGFIPA RDEVKPNEMI ALDRWAVSCA LEAQNDIIEA 700
    YEHYQFHTVV QRLMRFCSIE MGSFYLDIIK DRQYTTKADS LARRSCQTAL 750
    WHIAEALVRW IAPILSFTAD EIWSHLPTVE GRSEFVFTEQ FYSQLFTLNC 800
    HDKLDDNYWQ QLINIRAEVN RVLEQARNEK TIGAGLEAKV TVYANDEMLP 850
    LLHQLGNELR FVLITSQAIV KPLSEADVAE GELAGLAVKV ERAEGEKCPR 900
    CWHFATDIGT HAEHNAICGR CVENVAGKGE VRRFA 935
    Length:935
    Mass (Da):105,766
    Last modified:October 1, 2003 - v1
    Checksum:i7050B5D1ACFFAF54
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017143 Genomic DNA. Translation: AAP95253.1.
    RefSeqiNP_872864.1. NC_002940.2.
    WP_010944306.1. NC_002940.2.

    Genome annotation databases

    EnsemblBacteriaiAAP95253; AAP95253; HD_0272.
    GeneIDi1490272.
    KEGGihdu:HD0272.
    PATRICi20177107. VBIHaeDuc133973_0221.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017143 Genomic DNA. Translation: AAP95253.1 .
    RefSeqi NP_872864.1. NC_002940.2.
    WP_010944306.1. NC_002940.2.

    3D structure databases

    ProteinModelPortali Q7VP34.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233412.HD0272.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP95253 ; AAP95253 ; HD_0272 .
    GeneIDi 1490272.
    KEGGi hdu:HD0272.
    PATRICi 20177107. VBIHaeDuc133973_0221.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci HDUC233412:GH5F-250-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Haemophilus ducreyi."
      Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 35000HP / ATCC 700724.

    Entry informationi

    Entry nameiSYI_HAEDU
    AccessioniPrimary (citable) accession number: Q7VP34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3