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Q7VP34

- SYI_HAEDU

UniProt

Q7VP34 - SYI_HAEDU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
Binding sitei603 – 6031ATPUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi901 – 9011ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHDUC233412:GH5F-250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HD_0272
OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
Taxonomic identifieri233412 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000001022: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 935935Isoleucine--tRNA ligasePRO_0000098394Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi233412.HD0272.

Structurei

3D structure databases

ProteinModelPortaliQ7VP34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VP34-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDYKDTLNL PETGFPMRGD LAKREPAMLK NWYDKNLYQQ IRQTSKGKKT
60 70 80 90 100
FILHDGPPYA NGNLHLGHAV NKILKDIIMK SKTASGFDTP YVPGWDCHGL
110 120 130 140 150
PIELKVESIV GKPNEKISAA EFRQACREYA NEQVDRQKAD FIRMGVLGDW
160 170 180 190 200
DNPYLTMNFN TEASIIRTLA KVIANGHLYK GSKPVHWCLD CGSSLAEAEV
210 220 230 240 250
EYEDKVSPSI YVRFKACDET AIETLFNAKG NGPISAIIWT TTPWTMPSNR
260 270 280 290 300
AIAIHPELEY ALVQLADERV ILATELVESV AKAIEAESFD ILATVKGETL
310 320 330 340 350
QLLRFHHPFY AFDVPFIFGD HVTTEGGTGL VHTAPDHGTD DFIIARKNNI
360 370 380 390 400
EMAGLIGNDG KFKSDVEFFA GLAVFESNEK VIEKLKETGA LLKLARIKHS
410 420 430 440 450
YPHCWRHKTP IIFRATPQWF IGMEKQGLRA QALAEIKTVR WIPNWGEARI
460 470 480 490 500
DTMVANRPDW CISRQRTWGV PMAMFVHNET EELHPRTLDI LELVAQRVEQ
510 520 530 540 550
QGIQAWWDLD PTEVLGEDAQ HYHKVPDTLD VWFDSGSTYA SVVEQRPEFN
560 570 580 590 600
GQTADMYLEG SDQHRGWFMS SLMLASATNG KAPYQQVLTH GFVVDEKGRK
610 620 630 640 650
MSKSIGNVIV PSEVWNKNGA DILRLWVAST DYTAEMKVSH GILNSAGDAY
660 670 680 690 700
RRIRNTARFL LSNLNGFIPA RDEVKPNEMI ALDRWAVSCA LEAQNDIIEA
710 720 730 740 750
YEHYQFHTVV QRLMRFCSIE MGSFYLDIIK DRQYTTKADS LARRSCQTAL
760 770 780 790 800
WHIAEALVRW IAPILSFTAD EIWSHLPTVE GRSEFVFTEQ FYSQLFTLNC
810 820 830 840 850
HDKLDDNYWQ QLINIRAEVN RVLEQARNEK TIGAGLEAKV TVYANDEMLP
860 870 880 890 900
LLHQLGNELR FVLITSQAIV KPLSEADVAE GELAGLAVKV ERAEGEKCPR
910 920 930
CWHFATDIGT HAEHNAICGR CVENVAGKGE VRRFA
Length:935
Mass (Da):105,766
Last modified:October 1, 2003 - v1
Checksum:i7050B5D1ACFFAF54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017143 Genomic DNA. Translation: AAP95253.1.
RefSeqiNP_872864.1. NC_002940.2.

Genome annotation databases

EnsemblBacteriaiAAP95253; AAP95253; HD_0272.
GeneIDi1490272.
KEGGihdu:HD0272.
PATRICi20177107. VBIHaeDuc133973_0221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017143 Genomic DNA. Translation: AAP95253.1 .
RefSeqi NP_872864.1. NC_002940.2.

3D structure databases

ProteinModelPortali Q7VP34.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233412.HD0272.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP95253 ; AAP95253 ; HD_0272 .
GeneIDi 1490272.
KEGGi hdu:HD0272.
PATRICi 20177107. VBIHaeDuc133973_0221.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HDUC233412:GH5F-250-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Haemophilus ducreyi."
    Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 35000HP / ATCC 700724.

Entry informationi

Entry nameiSYI_HAEDU
AccessioniPrimary (citable) accession number: Q7VP34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3