SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q7VP34

- SYI_HAEDU

UniProt

Q7VP34 - SYI_HAEDU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, HD_0272
Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylate By similarity
Binding sitei603 – 6031ATP By similarity
Metal bindingi898 – 8981Zinc By similarity
Metal bindingi901 – 9011Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity
Metal bindingi921 – 9211Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHDUC233412:GH5F-250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:HD_0272
OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
Taxonomic identifieri233412 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000001022: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 935935Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098394Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi233412.HD0272.

Structurei

3D structure databases

ProteinModelPortaliQ7VP34.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi600 – 6045"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VP34-1 [UniParc]FASTAAdd to Basket

« Hide

MTDYKDTLNL PETGFPMRGD LAKREPAMLK NWYDKNLYQQ IRQTSKGKKT    50
FILHDGPPYA NGNLHLGHAV NKILKDIIMK SKTASGFDTP YVPGWDCHGL 100
PIELKVESIV GKPNEKISAA EFRQACREYA NEQVDRQKAD FIRMGVLGDW 150
DNPYLTMNFN TEASIIRTLA KVIANGHLYK GSKPVHWCLD CGSSLAEAEV 200
EYEDKVSPSI YVRFKACDET AIETLFNAKG NGPISAIIWT TTPWTMPSNR 250
AIAIHPELEY ALVQLADERV ILATELVESV AKAIEAESFD ILATVKGETL 300
QLLRFHHPFY AFDVPFIFGD HVTTEGGTGL VHTAPDHGTD DFIIARKNNI 350
EMAGLIGNDG KFKSDVEFFA GLAVFESNEK VIEKLKETGA LLKLARIKHS 400
YPHCWRHKTP IIFRATPQWF IGMEKQGLRA QALAEIKTVR WIPNWGEARI 450
DTMVANRPDW CISRQRTWGV PMAMFVHNET EELHPRTLDI LELVAQRVEQ 500
QGIQAWWDLD PTEVLGEDAQ HYHKVPDTLD VWFDSGSTYA SVVEQRPEFN 550
GQTADMYLEG SDQHRGWFMS SLMLASATNG KAPYQQVLTH GFVVDEKGRK 600
MSKSIGNVIV PSEVWNKNGA DILRLWVAST DYTAEMKVSH GILNSAGDAY 650
RRIRNTARFL LSNLNGFIPA RDEVKPNEMI ALDRWAVSCA LEAQNDIIEA 700
YEHYQFHTVV QRLMRFCSIE MGSFYLDIIK DRQYTTKADS LARRSCQTAL 750
WHIAEALVRW IAPILSFTAD EIWSHLPTVE GRSEFVFTEQ FYSQLFTLNC 800
HDKLDDNYWQ QLINIRAEVN RVLEQARNEK TIGAGLEAKV TVYANDEMLP 850
LLHQLGNELR FVLITSQAIV KPLSEADVAE GELAGLAVKV ERAEGEKCPR 900
CWHFATDIGT HAEHNAICGR CVENVAGKGE VRRFA 935
Length:935
Mass (Da):105,766
Last modified:October 1, 2003 - v1
Checksum:i7050B5D1ACFFAF54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017143 Genomic DNA. Translation: AAP95253.1.
RefSeqiNP_872864.1. NC_002940.2.
WP_010944306.1. NC_002940.2.

Genome annotation databases

EnsemblBacteriaiAAP95253; AAP95253; HD_0272.
GeneIDi1490272.
KEGGihdu:HD0272.
PATRICi20177107. VBIHaeDuc133973_0221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017143 Genomic DNA. Translation: AAP95253.1 .
RefSeqi NP_872864.1. NC_002940.2.
WP_010944306.1. NC_002940.2.

3D structure databases

ProteinModelPortali Q7VP34.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233412.HD0272.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP95253 ; AAP95253 ; HD_0272 .
GeneIDi 1490272.
KEGGi hdu:HD0272.
PATRICi 20177107. VBIHaeDuc133973_0221.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HDUC233412:GH5F-250-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Haemophilus ducreyi."
    Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 35000HP / ATCC 700724.

Entry informationi

Entry nameiSYI_HAEDU
AccessioniPrimary (citable) accession number: Q7VP34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: September 3, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi