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Protein

Ribosomal RNA large subunit methyltransferase K/L

Gene

rlmL

Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA.UniRule annotation
S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA.UniRule annotation

GO - Molecular functioni

  1. 23S rRNA (guanine(2445)-N(2))-methyltransferase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: UniProtKB-HAMAP
  3. rRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHDUC233412:GH5F-284-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase K/LUniRule annotation
Including the following 2 domains:
23S rRNA m2G2445 methyltransferaseUniRule annotation (EC:2.1.1.173UniRule annotation)
Alternative name(s):
rRNA (guanine-N(2)-)-methyltransferase RlmLUniRule annotation
23S rRNA m7G2069 methyltransferaseUniRule annotation (EC:2.1.1.264UniRule annotation)
Alternative name(s):
rRNA (guanine-N(7)-)-methyltransferase RlmKUniRule annotation
Gene namesi
Name:rlmLUniRule annotation
Ordered Locus Names:HD_0307
OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
Taxonomic identifieri233412 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000001022: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 712712Ribosomal RNA large subunit methyltransferase K/LPRO_0000366759Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi233412.HD0307.

Structurei

3D structure databases

ProteinModelPortaliQ7VP04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 157112THUMPUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RlmKL family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0116.
KOiK12297.
OrthoDBiEOG6HJ238.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
HAMAPiMF_01858. 23SrRNA_methyltr_KL.
InterProiIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP.
[Graphical view]
PfamiPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFiPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VP04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNQITYFAT AARGFEEMLK IELEQICGAD GKVVQGGVHF TTNQKGAYQA
60 70 80 90 100
LLHSRLASRI LLPLVSTKIF SDLDLYATII AINWADIFDP RDTFYVDFNG
110 120 130 140 150
TNREIRNTQF GAMRVKDGIV DYFERKRFAR PIVDKDRPDI RIHVYLDREK
160 170 180 190 200
LIVSLDLSGE ALHMRGYRED TGKAPLRETL AAIIVLRSGW QKGTPLVDPM
210 220 230 240 250
CGSGTLLIEA AQMQAGIVPQ LQRKYWGFNA WKGHQQAIWQ QVLEEAHSQK
260 270 280 290 300
NTQIDPLFYG FDLDHRVLAK AKQNAQNAGV AHLIHWQQAD IAALKNPCLD
310 320 330 340 350
QKGTLVSNPP YGERLGTTPA LIALYSVFGQ RLKQQFAGWN VSIFSGEPSL
360 370 380 390 400
LNCLRLRSTR QFKAKNGPLD CLQKNYQIAE YAIHNQHISE SSVAQNTQVA
410 420 430 440 450
PDFANRLTKN IKKIEKWAKQ QQLDAYRLYD ADLPEYNFAV DRYSDHIVIQ
460 470 480 490 500
EYAAPKSIEQ NKARQRLLDA VTATLHVTGI ETNKLVLKVR QKQKGTNQYE
510 520 530 540 550
KLANKGEYFY VNEYGAKLWV NLTDYLDTGI FLDHRLTRKM VGQMAKGKTF
560 570 580 590 600
LNLFAYTGSA TIHAALHGAK ATTSVDMSNT YLNWAEQNLE LNGLKSRNHR
610 620 630 640 650
LFQADCLQWL AECRERFELI FVDPPTFSNS KRMEDSWDVQ RDHIKLMTQL
660 670 680 690 700
KRILTSDGMI VFSNNKRGFK MDFNGLTALG LVAENISYKT LPLDFERDPH
710
IHNCWIIRHI EN
Length:712
Mass (Da):81,310
Last modified:March 24, 2009 - v2
Checksum:iF035F3E436AA0BAB
GO

Sequence cautioni

The sequence AAP95286.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017143 Genomic DNA. Translation: AAP95286.1. Different initiation.
RefSeqiNP_872897.1. NC_002940.2.

Genome annotation databases

EnsemblBacteriaiAAP95286; AAP95286; HD_0307.
GeneIDi1490306.
KEGGihdu:HD0307.
PATRICi20177169. VBIHaeDuc133973_0252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017143 Genomic DNA. Translation: AAP95286.1. Different initiation.
RefSeqiNP_872897.1. NC_002940.2.

3D structure databases

ProteinModelPortaliQ7VP04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233412.HD0307.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP95286; AAP95286; HD_0307.
GeneIDi1490306.
KEGGihdu:HD0307.
PATRICi20177169. VBIHaeDuc133973_0252.

Phylogenomic databases

eggNOGiCOG0116.
KOiK12297.
OrthoDBiEOG6HJ238.

Enzyme and pathway databases

BioCyciHDUC233412:GH5F-284-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
HAMAPiMF_01858. 23SrRNA_methyltr_KL.
InterProiIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP.
[Graphical view]
PfamiPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFiPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Haemophilus ducreyi."
    Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 35000HP / ATCC 700724.

Entry informationi

Entry nameiRLMKL_HAEDU
AccessioniPrimary (citable) accession number: Q7VP04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: January 7, 2015
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.