Q7VP04 (RLMKL_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase K/L

Including the following 2 domains:

1. 23S rRNA m2G2445 methyltransferase
   EC=2.1.1.173
   Alternative name(s):
   rRNA (guanine-N(2)-)-methyltransferase RlmL
2. 23S rRNA m7G2069 methyltransferase
   EC=2.1.1.264
   Alternative name(s):
   rRNA (guanine-N(7)-)-methyltransferase RlmK

Gene names

Name:	rlmL
Ordered Locus Names:	HD_0307

Organism

Haemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]

Taxonomic identifier

233412 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus ducreyi

Protein attributes

Sequence length	712 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA By similarity. HAMAP-Rule MF_01858
Catalytic activity	S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA. HAMAP-Rule MF_01858 S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA. HAMAP-Rule MF_01858
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01858.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmKL family. Contains 1 THUMP domain.
Sequence caution	The sequence AAP95286.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	23S rRNA (guanine(2445)-N(2))-methyltransferase activity Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: HAMAP rRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 712	712	Ribosomal RNA large subunit methyltransferase K/L HAMAP-Rule MF_01858		PRO_0000366759
Regions
	Domain	46 – 157	112	THUMP

Sequences

	Sequence		Length	Mass (Da)
	Q7VP04 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: F035F3E436AA0BAB	FASTA	712	81,310
	10 20 30 40 50 60 MSNQITYFAT AARGFEEMLK IELEQICGAD GKVVQGGVHF TTNQKGAYQA LLHSRLASRI 70 80 90 100 110 120 LLPLVSTKIF SDLDLYATII AINWADIFDP RDTFYVDFNG TNREIRNTQF GAMRVKDGIV 130 140 150 160 170 180 DYFERKRFAR PIVDKDRPDI RIHVYLDREK LIVSLDLSGE ALHMRGYRED TGKAPLRETL 190 200 210 220 230 240 AAIIVLRSGW QKGTPLVDPM CGSGTLLIEA AQMQAGIVPQ LQRKYWGFNA WKGHQQAIWQ 250 260 270 280 290 300 QVLEEAHSQK NTQIDPLFYG FDLDHRVLAK AKQNAQNAGV AHLIHWQQAD IAALKNPCLD 310 320 330 340 350 360 QKGTLVSNPP YGERLGTTPA LIALYSVFGQ RLKQQFAGWN VSIFSGEPSL LNCLRLRSTR 370 380 390 400 410 420 QFKAKNGPLD CLQKNYQIAE YAIHNQHISE SSVAQNTQVA PDFANRLTKN IKKIEKWAKQ 430 440 450 460 470 480 QQLDAYRLYD ADLPEYNFAV DRYSDHIVIQ EYAAPKSIEQ NKARQRLLDA VTATLHVTGI 490 500 510 520 530 540 ETNKLVLKVR QKQKGTNQYE KLANKGEYFY VNEYGAKLWV NLTDYLDTGI FLDHRLTRKM 550 560 570 580 590 600 VGQMAKGKTF LNLFAYTGSA TIHAALHGAK ATTSVDMSNT YLNWAEQNLE LNGLKSRNHR 610 620 630 640 650 660 LFQADCLQWL AECRERFELI FVDPPTFSNS KRMEDSWDVQ RDHIKLMTQL KRILTSDGMI 670 680 690 700 710 VFSNNKRGFK MDFNGLTALG LVAENISYKT LPLDFERDPH IHNCWIIRHI EN

References

[1]

"The complete genome sequence of Haemophilus ducreyi." Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017143 Genomic DNA. Translation: AAP95286.1. Different initiation.
RefSeq	NP_872897.1. NC_002940.2.
3D structure databases
ProteinModelPortal	Q7VP04.
ModBase	Search...
Protein-protein interaction databases
STRING	233412.HD0307.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP95286; AAP95286; HD_0307.
GeneID	1490306.
KEGG	hdu:HD0307.
PATRIC	20177169. VBIHaeDuc133973_0252.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0116.
KO	K12297.
ProtClustDB	PRK11783.
Enzyme and pathway databases
BioCyc	HDUC233412:GH5F-272-MONOMER.
Family and domain databases
HAMAP	MF_01858. 23SrRNA_methyltr_KL.
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR000241. RNA_methylase_dom. IPR017244. S-Ado-dep_RNA_MeTrfase_bac. IPR019614. SAM-dep_methyl-trfase. IPR004114. THUMP. [Graphical view]
Pfam	PF10672. Methyltrans_SAM. 1 hit. PF02926. THUMP. 1 hit. PF01170. UPF0020. 1 hit. [Graphical view]
PIRSF	PIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMART	SM00981. THUMP. 1 hit. [Graphical view]
PROSITE	PS51165. THUMP. 1 hit. PS01261. UPF0020. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLMKL_HAEDU

Accession

Primary (citable) accession number: Q7VP04

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program