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Reviewed, UniProtKB/Swiss-Prot Q7VNX8 (NRFA_HAEDU)

Last modified February 9, 2010. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: HD_0344
OrganismHaemophilus ducreyi [Complete proteome] [HAMAP]
Taxonomic identifier730 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

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Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity. HAMAP MF_01182

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity. HAMAP MF_01182

Binds 5 heme groups covalently per monomer By similarity. HAMAP MF_01182

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity HAMAP MF_01182.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 502477Cytochrome c-552 HAMAP MF_01182
PRO_0000268966

Sites

Metal binding1051Iron (heme 3 axial ligand) By similarity
Metal binding1371Iron (heme 1 axial ligand) By similarity
Metal binding1751Iron (heme 2 axial ligand) By similarity
Metal binding2241Iron (heme 3 axial ligand) By similarity
Metal binding2261Calcium By similarity
Metal binding2271Calcium; via carbonyl oxygen By similarity
Metal binding2711Calcium; via carbonyl oxygen By similarity
Metal binding2731Calcium By similarity
Metal binding2851Iron (heme 5 axial ligand) By similarity
Metal binding2961Iron (heme 4 axial ligand) By similarity
Metal binding3111Iron (heme 2 axial ligand) By similarity
Metal binding3281Iron (heme 5 axial ligand) By similarity
Metal binding4031Iron (heme 4 axial ligand) By similarity
Binding site1331Heme 1 (covalent) By similarity
Binding site1361Heme 1 (covalent) By similarity
Binding site1711Heme 2 (covalent) By similarity
Binding site1741Heme 2 (covalent) By similarity
Binding site2201Heme 3 (covalent) By similarity
Binding site2231Heme 3 (covalent) By similarity
Binding site2271Substrate By similarity
Binding site2741Substrate By similarity
Binding site2921Heme 4 (covalent) By similarity
Binding site2951Heme 4 (covalent) By similarity
Binding site3241Heme 5 (covalent) By similarity
Binding site3271Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q7VNX8-1 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: FF214013B33FDE04

FASTA50256,641
        10         20         30         40         50         60 
MKYLTKSRVI ATIAMLGCLS VSAWAETPTN QPESLTDKAL KHEKLGVVVE SANHKFAEKY 

        70         80         90        100        110        120 
RLQYDSWKAT AESTDRSSAL EADPRLVVLW AGYAFAKEYN KPRGHYYAVT DVREILRTGA 

       130        140        150        160        170        180 
PKDENDGPQP MACWTCKGPD VPRLIEEKGE RGYFDPKWAK YGAEIVNSIG CADCHDTTSK 

       190        200        210        220        230        240 
AFEEGKPALR VARPHVLRAL ESVGWRFEDL DKHGKRVAVC SNCHVEYYFK DKKDVTFPWA 

       250        260        270        280        290        300 
KGVDVDSIEK YYDESQFTDW THALSKAPML KTQHPDFEVW SQGVHGKNGV TCIDCHMPKV 

       310        320        330        340        350        360 
KDKDGKVYTE HKIGNPFDRF DATCKTCHEQ SKQTLQDRVK EHKAQVKEAM IRLEDQIVKA 

       370        380        390        400        410        420 
HFEAKVAWDA GATAEEMQDI LMAIRHAQWR WDYSAAGHGN HFHAPDVMLH TIATGLDRVA 

       430        440        450        460        470        480 
DARAKLGVVL TKHGVETPIV MPDISTREKA QKAVGIDIAK DQAAKDEFLR TVVPQWERQA 

       490        500 
RERGLLPEVT PKSVTTPKVD AK

« Hide

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References

[1]"The complete genome sequence of Haemophilus ducreyi."
Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 35000HP / ATCC 700724.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017143 Genomic DNA. Translation: AAP95319.1. Different initiation.
RefSeqNP_872930.1.

3D structure databases

HSSPHSSP built from PDB template 1FS7 based on UniProtKB Q9S1E5.
SMRQ7VNX8. Positions 49-488.
ModBaseSearch...

Genome annotation databases

GeneID1490348.
GenomeReviewsGene locus HD_0344 in contig AE017143_GR.
KEGGhdu:HD0344.
NMPDRfig|233412.1.peg.295.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG488281.

Enzyme and pathway databases

BioCycHDUC233412:HD_0344-MONOMER.
BRENDA1.7.2.2. 265031.

Family and domain databases

HAMAPMF_01182. Cytochrom_C552.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_HAEDU
AccessionPrimary (citable) accession number: Q7VNX8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: February 9, 2010
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents