Reviewed, UniProtKB/Swiss-Prot Q7VNI5 (PLSB_HAEDU)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	HD_0546

Organism

Haemophilus ducreyi [Complete proteome] [HAMAP]

Taxonomic identifier

730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	811 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 811	811	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_0000195221
Regions
	Motif	303 – 308	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q7VNI5-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 8F449267F8B77E2C	FASTA	811	92,885
	10 20 30 40 50 60 MSSLLSFYRN ILNFPLSLLV KSQAIPTDPV SELGLNLEQP IIYVLPYTSQ TDLLILQKNC 70 80 90 100 110 120 LALNLPDPLV ENDIQGQSLP RYVFLDEGHR FFKSKGVKSE TESLFYRYLD LHKTDETLDV 130 140 150 160 170 180 QLVPVSVLWG RSPGKETAPS LRLLSRFQRI IAMIWFGRDN FVRFSQAVSL CYMVKEYGAE 190 200 210 220 230 240 KGIAQKLARV AKIHFAKQRY SAMGPRLPER QAMFDKLIQL PTIVQAIEDE AKTKKIPIPK 250 260 270 280 290 300 ARQEAEKILD EIAADVSHGT LRMADRVLSW LWNKLYQGIN VQNADRVRKL ALEGHEIIYV 310 320 330 340 350 360 PCHRSHMDYL LLSYILYHQG VVPPHIAAGI NLNFWPAGPF FRRGGAFFIR RTFKGNRLYS 370 380 390 400 410 420 TVFREYLAEL FYRGYSVEYF IEGGRSRTGR LLEPKTGMVS MTLQALQRGL NRPISIVPVY 430 440 450 460 470 480 IGYEHVLEVD TYAKELRGAA KEKENAELVL RVIKKLRNLG QGYVNFGKPI QVNSYLNQHF 490 500 510 520 530 540 PEWKLPPVEN VRPKWLNEAV DAIAKQVMVN INNAAAVNAK NLIGSVLLAS RQRALSREQL 550 560 570 580 590 600 IEQVESYLQL FQNVSYSSDI ILPTESADEM LEHVLALPRS GVMSEKDNFG EMIRLDRESA 610 620 630 640 650 660 VLMTYYRNNI QHLFVLPSLV ASIVLHNEAA SKTLIRETVS HIYPFLKAEL FLHFDEKEVL 670 680 690 700 710 720 EQVELILTEF IRQQIVKYDG DVLTINRRRL PTLQLHAAGI REILQRYYIS LSLLLECPAI 730 740 750 760 770 780 SRTLLEKESR MIAQRLSILH GINAPEFFDK AIFSTFTASL KAQGYFDLEG HTVIEKVEEV 790 800 810 AHILRRLISV EVQLTIQGAM DKVDQIDEKL E

References

[1]

"The complete genome sequence of Haemophilus ducreyi." Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017143 Genomic DNA. Translation: AAP95485.1.
RefSeq	NP_873096.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1490523.
GenomeReviews	Gene locus HD_0546 in contig AE017143_GR.
KEGG	hdu:HD0546.
NMPDR	fig|233412.1.peg.461.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VNI5.
OMA	VPVYIGY.
Enzyme and pathway databases
BioCyc	HDUC233412:HD_0546-MON.
BRENDA	2.3.1.15. 265031.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_HAEDU

Accession

Primary (citable) accession number: Q7VNI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)