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Q7VN18 (TRMB_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:HD_0775
OrganismHaemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]
Taxonomic identifier233412 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length249 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 249249tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171332

Regions

Region228 – 2314Substrate binding By similarity

Sites

Active site1541 By similarity
Binding site791S-adenosyl-L-methionine By similarity
Binding site1041S-adenosyl-L-methionine By similarity
Binding site1311S-adenosyl-L-methionine By similarity
Binding site1541S-adenosyl-L-methionine By similarity
Binding site1581Substrate By similarity
Binding site1901Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VN18 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 2040841D405DC489

FASTA24928,597
        10         20         30         40         50         60 
MSEKYTFADQ KRKTVEQAEF TEDGRYLRKV RSFVLRTGRL SDFQRDMMND HWAKFGLDYQ 

        70         80         90        100        110        120 
KSPFDFVEIF GNANPVVLEI GFGMGRSLVE MAAQNPDRNY IGIEVHTPGV GACIAYAVEK 

       130        140        150        160        170        180 
QVDNLRVICH DATEILRDAV ADGSLAGLQL YFPDPWQKSK HHKRRIVQTE FVMRLLTKLT 

       190        200        210        220        230        240 
AGGFIHFATD WQNYAEHMLA VLRQFSPALV NTSPTGDFID RPAFRPLTKF EARGQRLGHA 


VWDLYFVKK 

« Hide

References

[1]"The complete genome sequence of Haemophilus ducreyi."
Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017143 Genomic DNA. Translation: AAP95680.1.
RefSeqNP_873291.1. NC_002940.2.

3D structure databases

ProteinModelPortalQ7VN18.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233412.HD0775.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP95680; AAP95680; HD_0775.
GeneID1490727.
KEGGhdu:HD0775.
PATRIC20177965. VBIHaeDuc133973_0633.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycHDUC233412:GH5F-705-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_HAEDU
AccessionPrimary (citable) accession number: Q7VN18
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2003
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways