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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791S-adenosyl-L-methionineUniRule annotation
Binding sitei104 – 1041S-adenosyl-L-methionineUniRule annotation
Binding sitei131 – 1311S-adenosyl-L-methionineUniRule annotation
Active sitei154 – 1541By similarity
Binding sitei154 – 1541S-adenosyl-L-methionineUniRule annotation
Binding sitei158 – 1581SubstrateUniRule annotation
Binding sitei190 – 1901SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHDUC233412:GH5F-705-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:HD_0775
OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
Taxonomic identifieri233412 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000001022: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249tRNA (guanine-N(7)-)-methyltransferasePRO_0000171332Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi233412.HD0775.

Structurei

3D structure databases

ProteinModelPortaliQ7VN18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni228 – 2314Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VN18-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEKYTFADQ KRKTVEQAEF TEDGRYLRKV RSFVLRTGRL SDFQRDMMND
60 70 80 90 100
HWAKFGLDYQ KSPFDFVEIF GNANPVVLEI GFGMGRSLVE MAAQNPDRNY
110 120 130 140 150
IGIEVHTPGV GACIAYAVEK QVDNLRVICH DATEILRDAV ADGSLAGLQL
160 170 180 190 200
YFPDPWQKSK HHKRRIVQTE FVMRLLTKLT AGGFIHFATD WQNYAEHMLA
210 220 230 240
VLRQFSPALV NTSPTGDFID RPAFRPLTKF EARGQRLGHA VWDLYFVKK
Length:249
Mass (Da):28,597
Last modified:October 1, 2003 - v1
Checksum:i2040841D405DC489
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017143 Genomic DNA. Translation: AAP95680.1.
RefSeqiNP_873291.1. NC_002940.2.

Genome annotation databases

EnsemblBacteriaiAAP95680; AAP95680; HD_0775.
GeneIDi1490727.
KEGGihdu:HD0775.
PATRICi20177965. VBIHaeDuc133973_0633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017143 Genomic DNA. Translation: AAP95680.1.
RefSeqiNP_873291.1. NC_002940.2.

3D structure databases

ProteinModelPortaliQ7VN18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233412.HD0775.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP95680; AAP95680; HD_0775.
GeneIDi1490727.
KEGGihdu:HD0775.
PATRICi20177965. VBIHaeDuc133973_0633.

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciHDUC233412:GH5F-705-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Haemophilus ducreyi."
    Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 35000HP / ATCC 700724.

Entry informationi

Entry nameiTRMB_HAEDU
AccessioniPrimary (citable) accession number: Q7VN18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2003
Last modified: February 4, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.