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Q7VMH0 (BIOB_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:HD_1007
OrganismHaemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]
Taxonomic identifier233412 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Biotin synthase HAMAP-Rule MF_01694
PRO_0000381413

Sites

Metal binding691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1131Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1441Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2041Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2761Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VMH0 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 34D7B9E9CC8EC83A

FASTA33437,349
        10         20         30         40         50         60 
MTTYNLRLKA KDELTPHPSV QYWRKCQVEE LFAMPFMELV FKAAQVHREH FDPQAIQLST 

        70         80         90        100        110        120 
LLSIKTGGCP EECEYCPQSA RYDTGLEKQV MLDIEEIVEK AKIAKQRGAS RFCMGAAWRG 

       130        140        150        160        170        180 
PKPKDIKVVS EIIGAVKALG LETCGTFGLL EDGMAEDLKQ AGLDYYNHNL DTDPERYSKV 

       190        200        210        220        230        240 
VHTRSHDDRM DTLGKVRHAG LKVCCGGIVG MNETRPERAG LIASLANLDP QPESVPINQL 

       250        260        270        280        290        300 
IKVEGTPMAE AEDLDWTEFV RTIAVARITM PKSYVRLSAG RTAMSEAMQT LCFIAGANSI 

       310        320        330 
FYGEKLLTTC NPEEDADCLL MQKLDLYPLQ YQEC 

« Hide

References

[1]"The complete genome sequence of Haemophilus ducreyi."
Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017143 Genomic DNA. Translation: AAP95886.1.
RefSeqNP_873497.1. NC_002940.2.

3D structure databases

ProteinModelPortalQ7VMH0.
SMRQ7VMH0. Positions 22-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233412.HD1007.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP95886; AAP95886; HD_1007.
GeneID1490947.
KEGGhdu:HD1007.
PATRIC20178377. VBIHaeDuc133973_0828.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2305620.

Enzyme and pathway databases

BioCycHDUC233412:GH5F-925-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_HAEDU
AccessionPrimary (citable) accession number: Q7VMH0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 1, 2003
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways