Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q7VM66 (SYL_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:HD_1132
OrganismHaemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]
Taxonomic identifier233412 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152022

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VM66 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: E70ED792F8BCFB3E

FASTA86198,050
        10         20         30         40         50         60 
MQQQYNPSAI ESKVQHFWEE NKVFKAVKDI TKEKYYCLSM LPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMIG KNVLQPMGWD AFGLPAEGAA VKNNTAPAKW TYENIEYMKN QLKVLGFSYD 

       130        140        150        160        170        180 
WDREITTCRP EYYKWEQWFF TELYKKGLVY KKTSTVNWCP NDETVLANEQ VHEGCCWRCD 

       190        200        210        220        230        240 
TPVEQRDIPQ WFIKITDYAE ELLTQLDNLT QWPDQVKTMQ RNWIGRSEGV EITFKIAGSN 

       250        260        270        280        290        300 
ATLPVYTTRP DTFFGVSYLA VAAAHPLAEL ASTNNPALAE FIREAKNTKV AEAELATMEK 

       310        320        330        340        350        360 
KGMPTGLFAI HPLTGKEIPV WVANFVLMHY GTGAVMAVPA HDERDFDFAK KYGLQINQVI 

       370        380        390        400        410        420 
QPLDASTWDF SQAAFTEHGK LINSAEFDGL DFNHAFNAIA DKLESLGVGK RQVNFRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMMTTEQG EVVTVPLKDL PVILPENVVM DGVQSPIKSD PEWAKTTYEG 

       490        500        510        520        530        540 
QPALKETDTF DTFMESSWYY ARYTCPQYHQ GMLDADEANY WLPVDQYIGG IEHATMHLLY 

       550        560        570        580        590        600 
FRFFHKLLRD AGILTSDEPA TKLLCQGMVL ADAFYYTSPT NERIWVSPTQ VSLERDEKGR 

       610        620        630        640        650        660 
IINATDPAGH KLVHSGMTKM SKSKNNGIDP QEMVEKYGAD TVRLFMMFAS PAEMTLEWQE 

       670        680        690        700        710        720 
SGVEGAKRFL GRVWNLVYEY VQQPATQALN ALELNKAQKE LRRDVHKTIA KVSDDIGRRQ 

       730        740        750        760        770        780 
TFNTAIAAIM ELMNKLNKAP LESDQDKAVM AEALSAVVRM LYPITPHICF ELWQALGNQQ 

       790        800        810        820        830        840 
TIDFAPWVVS DETAMIEDEK LVVVQVNGKV RGKITVPANA SEDEVKITAK NDANVAKFLA 

       850        860 
DMQIVKEIYI PFKMLNFVVK V 

« Hide

References

[1]"The complete genome sequence of Haemophilus ducreyi."
Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017143 Genomic DNA. Translation: AAP95994.1.
RefSeqNP_873605.1. NC_002940.2.

3D structure databases

ProteinModelPortalQ7VM66.
SMRQ7VM66. Positions 228-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233412.HD1132.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP95994; AAP95994; HD_1132.
GeneID1491059.
KEGGhdu:HD1132.
PATRIC20178605. VBIHaeDuc133973_0940.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycHDUC233412:GH5F-1037-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_HAEDU
AccessionPrimary (citable) accession number: Q7VM66
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries