Q7VLG6 (BIOD1_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD 1
EC=6.3.3.3

Alternative name(s):
DTB synthetase 1
Short name=DTBS 1
Dethiobiotin synthase 1

Gene names

Name:	bioD1
Ordered Locus Names:	HD_1480

Organism

Haemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]

Taxonomic identifier

233412 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	ATP-dependent dethiobiotin synthetase BioD 1 HAMAP MF_00336		PRO_0000187967
Regions
	Nucleotide binding	51 – 56	6	ATP By similarity
	Nucleotide binding	223 – 224	2	ATP By similarity
	Nucleotide binding	252 – 254	3	ATP By similarity
Sites
	Metal binding	51	1	Magnesium 1 By similarity
	Metal binding	55	1	Magnesium 2 By similarity
	Metal binding	102	1	Magnesium 2 By similarity
	Metal binding	163	1	Magnesium 2 By similarity
	Binding site	102	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VLG6 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 8F93B3069BC6EF99	FASTA	278	31,444
	10 20 30 40 50 60 MFSDKTRNLS RKFDQEHHYT LKKVLKYKQF CNYFVKRKKI PSLFITGTDT NVGKTIVTRA 70 80 90 100 110 120 ILQVLAQHNF SVVGYKPIAC GGDDDLPTEP NQTDYTSEDN SDVLIILDSC PQPVAYRDIN 130 140 150 160 170 180 SYTFTHSSTP IFAALDAVHH IQEEKLDTDL SNLQQNYPNV VVEGTYGWLT PINKDLSFAE 190 200 210 220 230 240 WVHKNNMPAV LVVGIKEGCV NHALLTAQAI QQQGVNLIGW VANRINPCLR HYAELIELLS 250 260 270 KKISAPLLGQ IPYIAQPHKK DLTSYIENPD PLLAYFQK

References

[1]

"The complete genome sequence of Haemophilus ducreyi." Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017143 Genomic DNA. Translation: AAP96282.1.
RefSeq	NP_873893.1. NC_002940.2.
3D structure databases
ProteinModelPortal	Q7VLG6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1491363.
GenomeReviews	Gene locus HD_1480 in contig AE017143_GR.
KEGG	hdu:HD1480.
NMPDR	fig|233412.1.peg.1258.
PATRIC	20179208. VBIHaeDuc133973_1237.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	INPGLSH.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	HDUC233412:HD_1480-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. Divergent sequence. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD1_HAEDU

Accession

Primary (citable) accession number: Q7VLG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program