Q7VL12 (BIOD2_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD 2
EC=6.3.3.3

Alternative name(s):
DTB synthetase 2
Short name=DTBS 2
Dethiobiotin synthase 2

Gene names

Name:	bioD2
Ordered Locus Names:	HD_1680

Organism

Haemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]

Taxonomic identifier

233412 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	ATP-dependent dethiobiotin synthetase BioD 2 HAMAP MF_00336		PRO_0000187968
Regions
	Nucleotide binding	13 – 18	6	ATP By similarity
	Nucleotide binding	115 – 118	4	ATP By similarity
	Nucleotide binding	175 – 176	2	ATP By similarity
Sites
	Metal binding	13	1	Magnesium 1 By similarity
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	50	1	Magnesium 2 By similarity
	Metal binding	115	1	Magnesium 2 By similarity
	Binding site	42	1	Substrate By similarity
	Binding site	50	1	ATP By similarity
	Binding site	211	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VL12 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 92DA228C4337A54C	FASTA	211	23,679
	10 20 30 40 50 60 MGKIIFVSGI DTDIGKTIAT GFYAKRLMQQ GFSVITQKMI QTGCQHISAD IIKHRQLQGI 70 80 90 100 110 120 ELTAEDLNGI TCPYLFRYPC SPHLAAEMEN NPILPSKIAQ ASALLAQKYD YVLLEGAGGL 130 140 150 160 170 180 AVPYNSQQTT LDYIVEQQLP LILVTSAKLG SINHTLLSLI VCQQHKIEME AVIYNTYPLE 190 200 210 DEKIAKSTQL YLQQYIKQHF PNTEFLVMDR Q

References

[1]

"The complete genome sequence of Haemophilus ducreyi." Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017143 Genomic DNA. Translation: AAP96447.1.
RefSeq	NP_874058.1. NC_002940.2.
3D structure databases
ProteinModelPortal	Q7VL12.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1491539.
GenomeReviews	Gene locus HD_1680 in contig AE017143_GR.
KEGG	hdu:HD1680.
NMPDR	fig|233412.1.peg.1423.
PATRIC	20179557. VBIHaeDuc133973_1407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	LNNYNHE.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	HDUC233412:HD_1680-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD2_HAEDU

Accession

Primary (citable) accession number: Q7VL12

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program