Q7VKJ0 (ASNA_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	HD_1913

Organism

Haemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]

Taxonomic identifier

233412 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195877

Sequences

	Sequence		Length	Mass (Da)
	Q7VKJ0 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 92AB16CE1AA98EE1	FASTA	330	37,325
	10 20 30 40 50 60 MKKSFILQQQ EISFVKNTFT QYLIDQLDII EVQGPILSQV GNGMQDNLSG IEKAVQVNVK 70 80 90 100 110 120 CIPGAVFEVV HSLAKWKRHT LARFGFKQGE GLFVHMKALR PDEDSLDPTH SIYVDQWDWE 130 140 150 160 170 180 KVIPEGQRNF AYLKQTVNQI YKAIRLTELA VEARFDIPSS LPKQITFIHS EELAQRYPNM 190 200 210 220 230 240 SSKERENAIC KEHGAIFLIG IGGKLSDGKA HDGRAPDYDD WTTESENGYK GLNGDILVWN 250 260 270 280 290 300 EELGSAFELS SMGIRVDQQA LRLQVSLTGD EDRLTMDWHQ DLLAGRLPLS IGGGIGQSRL 310 320 330 VMFLLHKKHI GEVQSSVWPT EMLTQFENIL

References

[1]

"The complete genome sequence of Haemophilus ducreyi." Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017143 Genomic DNA. Translation: AAP96637.1.
RefSeq	NP_874248.1. NC_002940.2.
3D structure databases
ProteinModelPortal	Q7VKJ0.
SMR	Q7VKJ0. Positions 4-330.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1491751.
GenomeReviews	Gene locus HD_1913 in contig AE017143_GR.
KEGG	hdu:HD1913.
NMPDR	fig|233412.1.peg.1613.
PATRIC	20179976. VBIHaeDuc133973_1604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	HDUC233412:HD_1913-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_HAEDU

Accession

Primary (citable) accession number: Q7VKJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program