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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187SubstrateUniRule annotation1
Active sitei188Proton donor/acceptorUniRule annotation1
Active sitei318UniRule annotation1
Binding sitei319SubstrateUniRule annotation1
Sitei331Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:HD_1986
OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
Taxonomic identifieri233412 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000001022 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001612781 – 464Fumarate hydratase class IIAdd BLAST464

Proteomic databases

PRIDEiQ7VKC9.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi233412.HD1986.

Structurei

3D structure databases

ProteinModelPortaliQ7VKC9.
SMRiQ7VKC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 100Substrate bindingUniRule annotation3
Regioni129 – 132Substrate binding (B site)UniRule annotation4
Regioni139 – 141Substrate bindingUniRule annotation3
Regioni324 – 326Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7VKC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYRIEKDTM GEVKVPADRY WAAQTERSRN NFRIGPEASM PVEIIEAFAY
60 70 80 90 100
LKKAAAFANA ELGALTTEKR DLIAMVCDEI LANKLADEFP LVIWQTGSGT
110 120 130 140 150
QSNMNLNEVI ANRAHVLQGG ALGEKSIIHP NDDVNKSQSS NDTYPTAMHI
160 170 180 190 200
AAYKKVVEVT IPAIERLQKT FAIKSEAFKD VVKIGRTHLM DATPLTLGQE
210 220 230 240 250
FSAYAAQLQF GLLALKNTLP HLAQLALGGT AVGTGLNTPK GYDVKVAEYI
260 270 280 290 300
AQFTQLPFIT AENKFEALAT HDAIIETHGA LKQIAMSLFK IANDIRLLAS
310 320 330 340 350
GPRSGIGEIL IPENEPGSSI MPGKVNPTQC EAMTMVAAQV LGNDTTISFA
360 370 380 390 400
GSQGHFQLNV FKPVMAANFL QSAQLLADVC ISFDEHCASG IEPNYPRIQQ
410 420 430 440 450
QLENSLMLVT ALNTHIGYEN AAKIAKTAHK NGTTLKEEAI NLGLVTAEQF
460
DQWVRPQNMV GSLK
Length:464
Mass (Da):50,483
Last modified:October 1, 2003 - v1
Checksum:i7FC2E242353C1DEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017143 Genomic DNA. Translation: AAP96703.1.
RefSeqiWP_010945724.1. NC_002940.2.

Genome annotation databases

EnsemblBacteriaiAAP96703; AAP96703; HD_1986.
GeneIDi24944683.
KEGGihdu:HD_1986.

Similar proteinsi

Entry informationi

Entry nameiFUMC_HAEDU
AccessioniPrimary (citable) accession number: Q7VKC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2003
Last modified: August 30, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families