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Q7VJT5 (NAPA_HELHP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:HH_0158
OrganismHelicobacter hepaticus (strain ATCC 51449 / 3B1) [Complete proteome] [HAMAP]
Taxonomic identifier235279 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length937 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4242Tat-type signal Potential
Chain43 – 937895Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045989

Regions

Domain49 – 110624Fe-4S Mo/W bis-MGD-type

Sites

Metal binding561Iron-sulfur (4Fe-4S) By similarity
Metal binding591Iron-sulfur (4Fe-4S) By similarity
Metal binding631Iron-sulfur (4Fe-4S) By similarity
Metal binding961Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VJT5 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: D89063CE3968E830

FASTA937105,424
        10         20         30         40         50         60 
MTSKIQGKKP TLSRRDFIKS AAAASAAASV GLSIPSVMSA EAQNAQKLWK WDKSVCRFCG 

        70         80         90        100        110        120 
TGCGIMVATQ KDNTGQAKIV AIKGDPEAPV NRGLNCIKGY FCAKIMYGAD RLTTPLLRVN 

       130        140        150        160        170        180 
SNGEFDKKGK FAPVSWKRAF DEMEKQFKKA YNELGPTGIA VFGSGQYTIQ EGYAAVKLVK 

       190        200        210        220        230        240 
GGFRSNNIDP NARHCMASAV VGFMETFGID EPAGCYDDIE LTDTIVTWGA NMAEMHPVLW 

       250        260        270        280        290        300 
SRVTDRKLSS SNVKVINLST YTNRTSDLAD IEIIFKPHTD LAIWNFLARE IINRNAVDEA 

       310        320        330        340        350        360 
FVKENCVFST GFVNIGYGMR NNPQHPKFKP EERDIVAKEV SKIVSNDEGI TLQYLGIKAG 

       370        380        390        400        410        420 
EEMKMDKAGA AGNHWGISFE DFKKGLEPYT LDFVANLAKG NPDESIESFK QKLQSLADYY 

       430        440        450        460        470        480 
IDKNRKIVSF WTMGMNQHQR GTWVNEQSYM VHMLLGKQAK PGSGAFSLTG QPSACGTARE 

       490        500        510        520        530        540 
VGTFSHRLPA DMVVANPKHR EITEKIWHLP SGTLNSKIGA PYLKIMRDLE DGNIKWAWVQ 

       550        560        570        580        590        600 
VNNPWQNTAN ANHWIAAARE QDNFIVVSEC YPGISAKVAD LILPTAMIYE KWGAYGNAER 

       610        620        630        640        650        660 
RTQHWKQQVV APGEAMPDIW QMAEFAKRFK LSEVWDKGYE ALDIKPVLES AKAMGYTEED 

       670        680        690        700        710        720 
TLFDVLFANK NAKNFSAQDA LLKNEFNTEV LGDSRNVEDG NGEAFKGYGF FIQKYLWEEY 

       730        740        750        760        770        780 
RQFGLGHAHD LADFDTYHRV RGLRWPVVNG KETQWRFNAK YDFYAQKLGN GKAFAFYGNK 

       790        800        810        820        830        840 
GKDMPAGSLN APSEEKVSID NKAKIFLRPY MDPCEMPDKE YPMWLCTGRV LEHWHSGTMT 

       850        860        870        880        890        900 
MRVPELYRAV PEALCYMHPD DANAQNLEQN QVVWVESRRG KVKAKLDLRG RNRPPKGLIY 

       910        920        930 
VPWFDENVFI NKVCLDATCP ISKQTDFKKC AVKVYKA 

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References

[1]"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus."
Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. expand/collapse author list , Shen Z., Weber J., Frosch M., Fox J.G.
Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017125 Genomic DNA. Translation: AAP76755.1.
RefSeqNP_859689.1. NC_004917.1.

3D structure databases

ProteinModelPortalQ7VJT5.
SMRQ7VJT5. Positions 49-936.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING235279.HH0158.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP76755; AAP76755; HH_0158.
GeneID1493213.
KEGGhhe:HH0158.
PATRIC20587728. VBIHelHep90276_0155.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
KOK02567.
OMAHPKTRVA.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycHHEP235279:GHUA-162-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_HELHP
AccessionPrimary (citable) accession number: Q7VJT5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families