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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Helicobacter hepaticus (strain ATCC 51449 / 3B1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi59Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi63Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi96Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei98Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei166Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei191Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei195Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei433Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei437Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei543Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei591Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei618Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei903Substrate; via amide nitrogenUniRule annotation1
Binding sitei911Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei928Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:HH_0158
OrganismiHelicobacter hepaticus (strain ATCC 51449 / 3B1)
Taxonomic identifieri235279 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000002495 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Tat-type signalUniRule annotationAdd BLAST42
ChainiPRO_000004598943 – 937Periplasmic nitrate reductaseUniRule annotationAdd BLAST895

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Proteomic databases

PRIDEiQ7VJT5

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi235279.HH0158

Structurei

3D structure databases

ProteinModelPortaliQ7VJT5
SMRiQ7VJT5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 1104Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni228 – 235Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni568 – 569Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni827 – 836Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
KOiK02567
OMAiTQHWRQQ
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 2 hits
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10518 TAT_signal, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7VJT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKIQGKKP TLSRRDFIKS AAAASAAASV GLSIPSVMSA EAQNAQKLWK
60 70 80 90 100
WDKSVCRFCG TGCGIMVATQ KDNTGQAKIV AIKGDPEAPV NRGLNCIKGY
110 120 130 140 150
FCAKIMYGAD RLTTPLLRVN SNGEFDKKGK FAPVSWKRAF DEMEKQFKKA
160 170 180 190 200
YNELGPTGIA VFGSGQYTIQ EGYAAVKLVK GGFRSNNIDP NARHCMASAV
210 220 230 240 250
VGFMETFGID EPAGCYDDIE LTDTIVTWGA NMAEMHPVLW SRVTDRKLSS
260 270 280 290 300
SNVKVINLST YTNRTSDLAD IEIIFKPHTD LAIWNFLARE IINRNAVDEA
310 320 330 340 350
FVKENCVFST GFVNIGYGMR NNPQHPKFKP EERDIVAKEV SKIVSNDEGI
360 370 380 390 400
TLQYLGIKAG EEMKMDKAGA AGNHWGISFE DFKKGLEPYT LDFVANLAKG
410 420 430 440 450
NPDESIESFK QKLQSLADYY IDKNRKIVSF WTMGMNQHQR GTWVNEQSYM
460 470 480 490 500
VHMLLGKQAK PGSGAFSLTG QPSACGTARE VGTFSHRLPA DMVVANPKHR
510 520 530 540 550
EITEKIWHLP SGTLNSKIGA PYLKIMRDLE DGNIKWAWVQ VNNPWQNTAN
560 570 580 590 600
ANHWIAAARE QDNFIVVSEC YPGISAKVAD LILPTAMIYE KWGAYGNAER
610 620 630 640 650
RTQHWKQQVV APGEAMPDIW QMAEFAKRFK LSEVWDKGYE ALDIKPVLES
660 670 680 690 700
AKAMGYTEED TLFDVLFANK NAKNFSAQDA LLKNEFNTEV LGDSRNVEDG
710 720 730 740 750
NGEAFKGYGF FIQKYLWEEY RQFGLGHAHD LADFDTYHRV RGLRWPVVNG
760 770 780 790 800
KETQWRFNAK YDFYAQKLGN GKAFAFYGNK GKDMPAGSLN APSEEKVSID
810 820 830 840 850
NKAKIFLRPY MDPCEMPDKE YPMWLCTGRV LEHWHSGTMT MRVPELYRAV
860 870 880 890 900
PEALCYMHPD DANAQNLEQN QVVWVESRRG KVKAKLDLRG RNRPPKGLIY
910 920 930
VPWFDENVFI NKVCLDATCP ISKQTDFKKC AVKVYKA
Length:937
Mass (Da):105,424
Last modified:October 1, 2003 - v1
Checksum:iD89063CE3968E830
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017125 Genomic DNA Translation: AAP76755.1
RefSeqiWP_011115001.1, NC_004917.1

Genome annotation databases

EnsemblBacteriaiAAP76755; AAP76755; HH_0158
KEGGihhe:HH_0158

Similar proteinsi

Entry informationi

Entry nameiNAPA_HELHP
AccessioniPrimary (citable) accession number: Q7VJT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2003
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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