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Q7VJT5

- NAPA_HELHP

UniProt

Q7VJT5 - NAPA_HELHP

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Helicobacter hepaticus (strain ATCC 51449 / 3B1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi59 – 591Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi63 – 631Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi96 – 961Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciHHEP235279:GHUA-162-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:HH_0158
    OrganismiHelicobacter hepaticus (strain ATCC 51449 / 3B1)
    Taxonomic identifieri235279 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000002495: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4242Tat-type signalUniRule annotationAdd
    BLAST
    Chaini43 – 937895Periplasmic nitrate reductasePRO_0000045989Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi235279.HH0158.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7VJT5.
    SMRiQ7VJT5. Positions 49-936.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini49 – 110624Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF10518. TAT_signal. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q7VJT5-1 [UniParc]FASTAAdd to Basket

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    MTSKIQGKKP TLSRRDFIKS AAAASAAASV GLSIPSVMSA EAQNAQKLWK    50
    WDKSVCRFCG TGCGIMVATQ KDNTGQAKIV AIKGDPEAPV NRGLNCIKGY 100
    FCAKIMYGAD RLTTPLLRVN SNGEFDKKGK FAPVSWKRAF DEMEKQFKKA 150
    YNELGPTGIA VFGSGQYTIQ EGYAAVKLVK GGFRSNNIDP NARHCMASAV 200
    VGFMETFGID EPAGCYDDIE LTDTIVTWGA NMAEMHPVLW SRVTDRKLSS 250
    SNVKVINLST YTNRTSDLAD IEIIFKPHTD LAIWNFLARE IINRNAVDEA 300
    FVKENCVFST GFVNIGYGMR NNPQHPKFKP EERDIVAKEV SKIVSNDEGI 350
    TLQYLGIKAG EEMKMDKAGA AGNHWGISFE DFKKGLEPYT LDFVANLAKG 400
    NPDESIESFK QKLQSLADYY IDKNRKIVSF WTMGMNQHQR GTWVNEQSYM 450
    VHMLLGKQAK PGSGAFSLTG QPSACGTARE VGTFSHRLPA DMVVANPKHR 500
    EITEKIWHLP SGTLNSKIGA PYLKIMRDLE DGNIKWAWVQ VNNPWQNTAN 550
    ANHWIAAARE QDNFIVVSEC YPGISAKVAD LILPTAMIYE KWGAYGNAER 600
    RTQHWKQQVV APGEAMPDIW QMAEFAKRFK LSEVWDKGYE ALDIKPVLES 650
    AKAMGYTEED TLFDVLFANK NAKNFSAQDA LLKNEFNTEV LGDSRNVEDG 700
    NGEAFKGYGF FIQKYLWEEY RQFGLGHAHD LADFDTYHRV RGLRWPVVNG 750
    KETQWRFNAK YDFYAQKLGN GKAFAFYGNK GKDMPAGSLN APSEEKVSID 800
    NKAKIFLRPY MDPCEMPDKE YPMWLCTGRV LEHWHSGTMT MRVPELYRAV 850
    PEALCYMHPD DANAQNLEQN QVVWVESRRG KVKAKLDLRG RNRPPKGLIY 900
    VPWFDENVFI NKVCLDATCP ISKQTDFKKC AVKVYKA 937
    Length:937
    Mass (Da):105,424
    Last modified:October 1, 2003 - v1
    Checksum:iD89063CE3968E830
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017125 Genomic DNA. Translation: AAP76755.1.
    RefSeqiNP_859689.1. NC_004917.1.
    WP_011115001.1. NC_004917.1.

    Genome annotation databases

    EnsemblBacteriaiAAP76755; AAP76755; HH_0158.
    GeneIDi1493213.
    KEGGihhe:HH0158.
    PATRICi20587728. VBIHelHep90276_0155.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017125 Genomic DNA. Translation: AAP76755.1 .
    RefSeqi NP_859689.1. NC_004917.1.
    WP_011115001.1. NC_004917.1.

    3D structure databases

    ProteinModelPortali Q7VJT5.
    SMRi Q7VJT5. Positions 49-936.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 235279.HH0158.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP76755 ; AAP76755 ; HH_0158 .
    GeneIDi 1493213.
    KEGGi hhe:HH0158.
    PATRICi 20587728. VBIHelHep90276_0155.

    Phylogenomic databases

    eggNOGi COG0243.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci HHEP235279:GHUA-162-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF10518. TAT_signal. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51449 / 3B1.

    Entry informationi

    Entry nameiNAPA_HELHP
    AccessioniPrimary (citable) accession number: Q7VJT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3