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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Helicobacter hepaticus (strain ATCC 51449 / 3B1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi59 – 591Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi63 – 631Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi96 – 961Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciHHEP235279:GHUA-162-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:HH_0158
OrganismiHelicobacter hepaticus (strain ATCC 51449 / 3B1)
Taxonomic identifieri235279 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000002495 Componenti: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4242Tat-type signalUniRule annotationAdd
BLAST
Chaini43 – 937895Periplasmic nitrate reductasePRO_0000045989Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi235279.HH0158.

Structurei

3D structure databases

ProteinModelPortaliQ7VJT5.
SMRiQ7VJT5. Positions 49-936.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 110624Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7VJT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKIQGKKP TLSRRDFIKS AAAASAAASV GLSIPSVMSA EAQNAQKLWK
60 70 80 90 100
WDKSVCRFCG TGCGIMVATQ KDNTGQAKIV AIKGDPEAPV NRGLNCIKGY
110 120 130 140 150
FCAKIMYGAD RLTTPLLRVN SNGEFDKKGK FAPVSWKRAF DEMEKQFKKA
160 170 180 190 200
YNELGPTGIA VFGSGQYTIQ EGYAAVKLVK GGFRSNNIDP NARHCMASAV
210 220 230 240 250
VGFMETFGID EPAGCYDDIE LTDTIVTWGA NMAEMHPVLW SRVTDRKLSS
260 270 280 290 300
SNVKVINLST YTNRTSDLAD IEIIFKPHTD LAIWNFLARE IINRNAVDEA
310 320 330 340 350
FVKENCVFST GFVNIGYGMR NNPQHPKFKP EERDIVAKEV SKIVSNDEGI
360 370 380 390 400
TLQYLGIKAG EEMKMDKAGA AGNHWGISFE DFKKGLEPYT LDFVANLAKG
410 420 430 440 450
NPDESIESFK QKLQSLADYY IDKNRKIVSF WTMGMNQHQR GTWVNEQSYM
460 470 480 490 500
VHMLLGKQAK PGSGAFSLTG QPSACGTARE VGTFSHRLPA DMVVANPKHR
510 520 530 540 550
EITEKIWHLP SGTLNSKIGA PYLKIMRDLE DGNIKWAWVQ VNNPWQNTAN
560 570 580 590 600
ANHWIAAARE QDNFIVVSEC YPGISAKVAD LILPTAMIYE KWGAYGNAER
610 620 630 640 650
RTQHWKQQVV APGEAMPDIW QMAEFAKRFK LSEVWDKGYE ALDIKPVLES
660 670 680 690 700
AKAMGYTEED TLFDVLFANK NAKNFSAQDA LLKNEFNTEV LGDSRNVEDG
710 720 730 740 750
NGEAFKGYGF FIQKYLWEEY RQFGLGHAHD LADFDTYHRV RGLRWPVVNG
760 770 780 790 800
KETQWRFNAK YDFYAQKLGN GKAFAFYGNK GKDMPAGSLN APSEEKVSID
810 820 830 840 850
NKAKIFLRPY MDPCEMPDKE YPMWLCTGRV LEHWHSGTMT MRVPELYRAV
860 870 880 890 900
PEALCYMHPD DANAQNLEQN QVVWVESRRG KVKAKLDLRG RNRPPKGLIY
910 920 930
VPWFDENVFI NKVCLDATCP ISKQTDFKKC AVKVYKA
Length:937
Mass (Da):105,424
Last modified:September 30, 2003 - v1
Checksum:iD89063CE3968E830
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017125 Genomic DNA. Translation: AAP76755.1.
RefSeqiNP_859689.1. NC_004917.1.

Genome annotation databases

EnsemblBacteriaiAAP76755; AAP76755; HH_0158.
KEGGihhe:HH0158.
PATRICi20587728. VBIHelHep90276_0155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017125 Genomic DNA. Translation: AAP76755.1.
RefSeqiNP_859689.1. NC_004917.1.

3D structure databases

ProteinModelPortaliQ7VJT5.
SMRiQ7VJT5. Positions 49-936.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235279.HH0158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP76755; AAP76755; HH_0158.
KEGGihhe:HH0158.
PATRICi20587728. VBIHelHep90276_0155.

Phylogenomic databases

eggNOGiCOG0243.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciHHEP235279:GHUA-162-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51449 / 3B1.

Entry informationi

Entry nameiNAPA_HELHP
AccessioniPrimary (citable) accession number: Q7VJT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2006
Last sequence update: September 30, 2003
Last modified: March 31, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.