Q7VJT5 (NAPA_HELHP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase
EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	HH_0158

Organism

Helicobacter hepaticus (strain ATCC 51449 / 3B1) [Complete proteome] [HAMAP]

Taxonomic identifier

235279 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter hepaticus

Protein attributes

Sequence length	937 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with NapB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 42	42	Tat-type signal Potential
	Chain	43 – 937	895	Periplasmic nitrate reductase HAMAP-Rule MF_01630		PRO_0000045989
Sites
	Metal binding	56	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	59	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	63	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	96	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VJT5 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D89063CE3968E830	FASTA	937	105,424
	10 20 30 40 50 60 MTSKIQGKKP TLSRRDFIKS AAAASAAASV GLSIPSVMSA EAQNAQKLWK WDKSVCRFCG 70 80 90 100 110 120 TGCGIMVATQ KDNTGQAKIV AIKGDPEAPV NRGLNCIKGY FCAKIMYGAD RLTTPLLRVN 130 140 150 160 170 180 SNGEFDKKGK FAPVSWKRAF DEMEKQFKKA YNELGPTGIA VFGSGQYTIQ EGYAAVKLVK 190 200 210 220 230 240 GGFRSNNIDP NARHCMASAV VGFMETFGID EPAGCYDDIE LTDTIVTWGA NMAEMHPVLW 250 260 270 280 290 300 SRVTDRKLSS SNVKVINLST YTNRTSDLAD IEIIFKPHTD LAIWNFLARE IINRNAVDEA 310 320 330 340 350 360 FVKENCVFST GFVNIGYGMR NNPQHPKFKP EERDIVAKEV SKIVSNDEGI TLQYLGIKAG 370 380 390 400 410 420 EEMKMDKAGA AGNHWGISFE DFKKGLEPYT LDFVANLAKG NPDESIESFK QKLQSLADYY 430 440 450 460 470 480 IDKNRKIVSF WTMGMNQHQR GTWVNEQSYM VHMLLGKQAK PGSGAFSLTG QPSACGTARE 490 500 510 520 530 540 VGTFSHRLPA DMVVANPKHR EITEKIWHLP SGTLNSKIGA PYLKIMRDLE DGNIKWAWVQ 550 560 570 580 590 600 VNNPWQNTAN ANHWIAAARE QDNFIVVSEC YPGISAKVAD LILPTAMIYE KWGAYGNAER 610 620 630 640 650 660 RTQHWKQQVV APGEAMPDIW QMAEFAKRFK LSEVWDKGYE ALDIKPVLES AKAMGYTEED 670 680 690 700 710 720 TLFDVLFANK NAKNFSAQDA LLKNEFNTEV LGDSRNVEDG NGEAFKGYGF FIQKYLWEEY 730 740 750 760 770 780 RQFGLGHAHD LADFDTYHRV RGLRWPVVNG KETQWRFNAK YDFYAQKLGN GKAFAFYGNK 790 800 810 820 830 840 GKDMPAGSLN APSEEKVSID NKAKIFLRPY MDPCEMPDKE YPMWLCTGRV LEHWHSGTMT 850 860 870 880 890 900 MRVPELYRAV PEALCYMHPD DANAQNLEQN QVVWVESRRG KVKAKLDLRG RNRPPKGLIY 910 920 930 VPWFDENVFI NKVCLDATCP ISKQTDFKKC AVKVYKA

References

[1]

"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus." Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. , Shen Z., Weber J., Frosch M., Fox J.G. Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017125 Genomic DNA. Translation: AAP76755.1.
RefSeq	NP_859689.1. NC_004917.1.
3D structure databases
ProteinModelPortal	Q7VJT5.
SMR	Q7VJT5. Positions 49-936.
ModBase	Search...
Protein-protein interaction databases
STRING	235279.HH0158.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP76755; AAP76755; HH_0158.
GeneID	1493213.
KEGG	hhe:HH0158.
PATRIC	20587728. VBIHelHep90276_0155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
KO	K02567.
OMA	IISAPYE.
ProtClustDB	PRK13532.
Enzyme and pathway databases
BioCyc	HHEP235279:GHUA-159-MONOMER.
Family and domain databases
Gene3D	2.40.40.20. 2 hits.
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. PF10518. TAT_signal. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_HELHP

Accession

Primary (citable) accession number: Q7VJT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program