Q7VJ31 (PROB_HELHP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	HH_0419

Organism

Helicobacter hepaticus (strain ATCC 51449 / 3B1) [Complete proteome] [HAMAP]

Taxonomic identifier

235279 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter hepaticus

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_0000109680
Regions
	Nucleotide binding	164 – 165	2	ATP By similarity
	Nucleotide binding	206 – 212	7	ATP By similarity
Sites
	Binding site	9	1	ATP By similarity
	Binding site	47	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	144	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VJ31 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 8E477F23E69F344F	FASTA	264	29,369
	10 20 30 40 50 60 MQKPRIVLKI GSSNLCNGKI IDKTQIKALA QIISELKMRF DVILVSSGAV ASGHTTLHID 70 80 90 100 110 120 RNTLQNKQAL ASIGQPLLME SYREALKDYA IPTAQLLLVW RDFDSRKNTT FAKDTIDTLL 130 140 150 160 170 180 AHNALPIINE NDTIATDEMV FGDNDRLGAY VTYYFGAKLL IILSDIDGYF DKNPHQYDDA 190 200 210 220 230 240 QILPIVHSIP SQALEQTHSP HGDFATGGIV TKLIAADFLL QRKCMMFLSH GRKLDVLRDF 250 260 LLHNKQSSGT LFCPADSQGI KTLI

References

[1]

"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus." Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. , Shen Z., Weber J., Frosch M., Fox J.G. Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017125 Genomic DNA. Translation: AAP77016.1.
RefSeq	NP_859950.1. NC_004917.1.
3D structure databases
ProteinModelPortal	Q7VJ31.
ModBase	Search...
Protein-protein interaction databases
STRING	235279.HH0419.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP77016; AAP77016; HH_0419.
GeneID	1493473.
KEGG	hhe:HH0419.
PATRIC	20588290. VBIHelHep90276_0424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
KO	K00931.
OMA	HIAINAR.
ProtClustDB	PRK12314.
Enzyme and pathway databases
BioCyc	HHEP235279:GHUA-420-MONOMER.
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_HELHP

Accession

Primary (citable) accession number: Q7VJ31

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program