Q7VIM2 (SYE2_HELHP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	HH_0582

Organism

Helicobacter hepaticus (strain ATCC 51449 / 3B1) [Complete proteome] [HAMAP]

Taxonomic identifier

235279 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000119575
Regions
	Motif	6 – 16	11	"HIGH" region HAMAP MF_00022_B
	Motif	231 – 235	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	234	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VIM2 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D46963489B27BFFA	FASTA	441	51,047
	10 20 30 40 50 60 MLRFAPSPTG DMHIGNLRAA IFNYIIAKQQ NQKFLIRIED TDIARNIVDK DKEILNLLNL 70 80 90 100 110 120 FGLLWDNLVY QSSNFERHRQ LAQHLISKDL AFYCYCSKEF LESKRLEAKE QKKPFRYDPS 130 140 150 160 170 180 WAEIEKSSNT KPVVRIRGAS EAISFEDAIK GTLRFEAHEI DSFVILKEDG IPTYNFACAI 190 200 210 220 230 240 DDMLYDISFI VRGEDHVSNT PRQILIHRAL GYDKVLGYAH LPIILGESGS KMSKRDNASS 250 260 270 280 290 300 VAWLLEEGFL PQAIMNYLIS MGNHTPTEVF KLQDAYNWFD IKNIAKSPVK FDIKRLRFLN 310 320 330 340 350 360 REHLKMLNEQ EFALLLDSKD SSIGALGKLH LQEASTLNEI RSKIERIFSP KCIAQNEEGE 370 380 390 400 410 420 NFENECKILY DILHQMIESY DESLNDYDTF KNALMAKSSL KGKKFFKPLR ILLTGQTQGL 430 440 ELSEIYPYLR FFLRDIVRLS K

References

[1]

"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus." Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. , Shen Z., Weber J., Frosch M., Fox J.G. Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed: 12810954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017125 Genomic DNA. Translation: AAP77179.1.
RefSeq	NP_860113.1. NC_004917.1.
3D structure databases
ProteinModelPortal	Q7VIM2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1493636.
GenomeReviews	Gene locus HH_0582 in contig AE017125_GR.
KEGG	hhe:HH0582.
NMPDR	fig|235279.1.peg.582.
PATRIC	20588618. VBIHelHep90276_0586.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	VAYRYDG.
ProtClustDB	PRK12410.
Enzyme and pathway databases
BioCyc	HHEP235279:HH_0582-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_HELHP

Accession

Primary (citable) accession number: Q7VIM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program