Reviewed, UniProtKB/Swiss-Prot Q7VIJ1 (PYRC_HELHP)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	HH_0614

Organism

Helicobacter hepaticus [Complete proteome] [HAMAP]

Taxonomic identifier

32025 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6. HAMAP MF_00219
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Dihydroorotase HAMAP MF_00219		PRO_0000325572
Sites
	Metal binding	14	1	Zinc 1 By similarity
	Metal binding	16	1	Zinc 1 By similarity
	Metal binding	97	1	Zinc 1; via carbamate group By similarity
	Metal binding	97	1	Zinc 2; via carbamate group By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	170	1	Zinc 2 By similarity
	Metal binding	242	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	97	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VIJ1-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D11C3F0BA743F204	FASTA	343	38,495
	10 20 30 40 50 60 MPQTLQLTNP MDMHLHLREG EMLSAILPFS ANPFSAAVVM PNLKTPITTT AQALAYKRQI 70 80 90 100 110 120 LSLCSTSFEP LMSIFLTPDL DKQELIRAKE AGIHILKLYP KGSTTGSEKG VKEMLCEKTL 130 140 150 160 170 180 HIFEIAQELG FILSIHGESN GFCMEREYEF LPIFAHIAQH FPRLRIIIEH MSDRRSLELI 190 200 210 220 230 240 EQYPNLYATL TLHHITMNLD DVLGGGINPH HFCKPMLKTK KDQQALLQAA LNAHPKVSFG 250 260 270 280 290 300 SDSAPHLESA KLNSKGAAGI FSAPILLPAL AEVFAFHNAL DCLQTFISNR AIANYKLKHF 310 320 330 340 PIKTITLERV ENPVPPYINT PLGHIIPLRA QTNLSWRIKE ENR

References

[1]

"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus." Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. , Shen Z., Weber J., Frosch M., Fox J.G. Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed: 12810954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017125 Genomic DNA. Translation: AAP77211.1.
RefSeq	NP_860145.1.
3D structure databases
HSSP	HSSP built from PDB template 1J79 based on UniProtKB P05020.
ModBase	Search...
Genome annotation databases
GeneID	1493668.
GenomeReviews	Gene locus HH_0614 in contig AE017125_GR.
KEGG	hhe:HH0614.
NMPDR	fig|235279.1.peg.614.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VIJ1.
OMA	Q7VIJ1. IMPNLVP.
Enzyme and pathway databases
BioCyc	HHEP235279:HH_0614-MON.
BRENDA	3.5.2.3. 279780.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_HELHP

Accession

Primary (citable) accession number: Q7VIJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)