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Q7VI24 (TRMB_HELHP) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:HH_0785
OrganismHelicobacter hepaticus (strain ATCC 51449 / 3B1) [Complete proteome] [HAMAP]
Taxonomic identifier235279 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171334

Sites

Binding site1251S-adenosyl-L-methionine By similarity
Binding site1501S-adenosyl-L-methionine By similarity
Binding site1771S-adenosyl-L-methionine By similarity
Binding site2031Substrate By similarity
Binding site2331Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VI24 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 76C6FB9B861941EF

FASTA39646,253
        10         20         30         40         50         60 
MPHFLASHIT LPPMPFTQEG YTFVYEAKQC NDSSQSLILV RYEDNEFFLR KAWRGNKNNA 

        70         80         90        100        110        120 
ILKCEKSTKT QPTGIIKNAL KILCAYQKNV ISHNLNKNTP RQNLQSPYFQ SMDFFLDFAK 

       130        140        150        160        170        180 
PCLIEIGFGS GRHLLHLAQS NPHFMCIGIE IHTPSIEQIL RQVQLLGLEN LYIINGDARI 

       190        200        210        220        230        240 
LLEILSSHIA QGIYVHFPVP WNKKHHRRIF SPKFFQESLR VLDNNGVLHL RSDDEIYFQD 

       250        260        270        280        290        300 
ALSLALQQES ISLEIHKNQQ EVITSKYEAR WKKQQKNIYD LKIFHTYKNT NNEKNVKNTQ 

       310        320        330        340        350        360 
KNFYFDKILR KNLDNYKNFP HKKIANDWFL HIDNLYCAGD IYVLALCFGD FNQPQSKFLQ 

       370        380        390 
IEFCNNVSAR YIGSNPIPTQ AAIKAHKYLI QILTQE 

« Hide

References

[1]"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus."
Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. expand/collapse author list , Shen Z., Weber J., Frosch M., Fox J.G.
Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017125 Genomic DNA. Translation: AAP77382.1.
RefSeqNP_860316.1. NC_004917.1.

3D structure databases

ProteinModelPortalQ7VI24.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING235279.HH0785.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP77382; AAP77382; HH_0785.
GeneID1492474.
KEGGhhe:HH0785.
PATRIC20589022. VBIHelHep90276_0784.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMASKYEARW.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycHHEP235279:GHUA-808-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_HELHP
AccessionPrimary (citable) accession number: Q7VI24
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2003
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways