Reviewed, UniProtKB/Swiss-Prot Q7VHS5 (CARA_HELHP)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	HH_0888

Organism

Helicobacter hepaticus [Complete proteome] [HAMAP]

Taxonomic identifier

32025 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112281
Regions
	Domain	192 – 378	187	Glutamine amidotransferase type-1
	Region	1 – 188	188	CPSase HAMAP MF_01209
Sites
	Active site	272	1	Nucleophile By similarity
	Active site	355	1	By similarity
	Active site	357	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VHS5-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 66BFFC8671B21B6F	FASTA	378	41,746
	10 20 30 40 50 60 MSILKEAYLY FANGLFFKAQ SFGSDGTFVG EVVFNTSMSG YQEVITDPSY SGQFIVFSMP 70 80 90 100 110 120 EIGIVGVNNQ DSESPRAACT GVILSSYNDF VSNFRSEQSL STYLKEHNIM GICGVDTRNL 130 140 150 160 170 180 IKMLTTQGAM MMIASTQITQ ESELKASLSR TPPIQDINFI KEVSTKTSYV HTDSTFDFTH 190 200 210 220 230 240 FAYGTSPNFK AKVVAIDFGA KRNILNELVA VGLEVEVIPH SFSAAEILSR FKAGEIQGVF 250 260 270 280 290 300 LSNGPGDPLV LHNEIEQIKQ LIQSDIPIFG ICLGHQLLSI AHGFPTYKLK FGHHGSNHPI 310 320 330 340 350 360 KNLQSGAVEI TAQNHNYCVP ESIAQIATIT HRNLFDNTIE GVRYKDKPIF SVQHHPEASP 370 GPREARILFE EFAKLCCK

References

[1]

"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus." Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. , Shen Z., Weber J., Frosch M., Fox J.G. Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed: 12810954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017125 Genomic DNA. Translation: AAP77485.1.
RefSeq	NP_860419.1.
3D structure databases
HSSP	HSSP built from PDB template 1CE8 based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1492577.
GenomeReviews	Gene locus HH_0888 in contig AE017125_GR.
KEGG	hhe:HH0888.
NMPDR	fig|235279.1.peg.888.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VHS5.
OMA	Q7VHS5. EADIPFF.
Enzyme and pathway databases
BioCyc	HHEP235279:HH_0888-MON.
BRENDA	6.3.5.5. 279780.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_HELHP

Accession

Primary (citable) accession number: Q7VHS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)